1. Home
    2. Journals
    3. Journal of Biosciences
    4. Volume 46
    5. All articles

    • Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/046/0074

    • Keywords

       

      Fission yeast; heterochromatin; mating-type loci; oxidative stress; Pap1; silencing

    • Abstract

       

      Gene silencing in S. pombe occurs by heterochromatin formation at the centromere (cen), mating-type (mat)and telomere loci. It is mediated by silencing factors including Swi6, Clr1-4, Rhp6 and Pol-alpha. RNAi pathwayalso plays a role in establishment of silencing at the mat and cen loci. Recently, the stress response factors, Atf1and Pcr1were shown to play an RNAi-independent role in silencing at the mat3 locus through a cis-actingAtf1-binding site located within the repression element REIII and recruitment of the silencing factors Clr3 andClr6. Another cis-acting site, named repression element REII abutting the mat2 locus, also establishes heterochromatinstructure through Clr5 and histone deacetylases but independently of H3-Lys9-methylation andRNAi. Here, we report the occurrence of binding sites for another oxidative response factor, the pombe AP1-like factor Pap1, at the mating-type, centromere and telomere loci. By genetic studies we show that these sitesplay a role in silencing at the outer repeats of centromeres as well as mating-type locus and this effect ismediated through Pap1 binding site and interaction with and recruitment of the HP1/Swi6. Importantly, pap1-Deltacells display a silencing defect even in absence of the oxidative stress. Such a role of Pap1 in heterochromatinformation may be evolutionarily conserved.

    • Author Affiliations

       

      ASHOK KUMAR1 2 JAGPREET SINGH NANDA1 3 SHARANJOT SAINI1 4 JAGMOHAN SINGH1 5

      1. Institute of Microbial Technology, Sector 39A, Chandigarh 160 036, India
      2. Present Address: Department of Basic Science, College of Horticulture and Forestry, Jhalawar Campus, Agriculture University Kota, Jhalrapatan 326 023, India
      3. Present Address: Cedars Sinai, Department of Hematology and Oncology, 8700 Beverly Boulevard, Los Angeles, CA 90048, USA
      4. Present Address: Department of Biochemistry and Molecular Biology, Augusta University, 1410 Laney Walker Blvd, Augusta, GA 30912, USA
      5. Present Address: Department of Biochemistry, Panjab University, Chandigarh 160 014, India

    • Dates

       
      Published
      20 July 2021

    • Supplementary Material

       

  • Journal of Biosciences | News

      Forthcoming Special issue.


    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

      Posted on April 12, 2023
      <

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2023-2024 Indian Academy of Sciences, Bengaluru.

Contact | Site index