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      https://www.ias.ac.in/article/fulltext/jbsc/046/0065

    • Keywords

       

      Pseudomonas aeruginosa; dendritic cells; long noncoding RNAs; NRIR

    • Abstract

       

      N-3-(oxododecanoyl)-L-homoserine lactone (3-O-C12-HSL), a small bacterial signaling molecule secreted byPseudomonas aeruginosa (P. aeruginosa), can block dendritic cell (DC) maturation and participate in immuneescape, but the underlying mechanism is unclear. We speculate that regulation of DC maturation and functionby lncRNAs may be the mechanism by which 3-O-C12-HSL inhibits the immune response. We found that3-O-C12-HSL increased the expression level of the lncRNA NRIR, impeding monocyte-derived dendritic cell(Mo-DC) maturation. In addition, we observed the effect of NRIR on the expression of CD40, CD80, HLA-DRand IL-6. NRIR overexpression significantly reduced the expression of Mo-DC surface markers, while 3-OC12-HSL did not significantly reduce the expression of Mo-DC surface markers after NRIR knockdown.These results indicate that 3-O-C12-HSL indeed affects the differentiation and maturation of Mo-DCs throughNRIR. IL-6 stimulates T cell proliferation and activation, and we found that high NRIR expression reduced IL-6 levels. However, under NRIR knockdown, 3-O-C12-HSL did not decrease IL-6 expression, suggesting that3-O-C12-HSL may affect T cell activation through NRIR. This study is the first to elucidate the important roleof a lncRNA in the mechanism of 3-O-C12-HSL activity. It also provides new ideas regarding P. aeruginosainfection pathogenesis.

    • Author Affiliations

       

      XUAN ZHANG1 2 YANG LIU3 YANG LU1 2 SONG LI1 2 JIANPING LIU1 2 YUNYAN ZHANG4 LINA WANG1 2 MO LI1 2 YANFEN LUO1 2 WEIZHENG ZHANG1 2 CHA CHEN1 2 YOUQIANG LI1 2 5

      1. Department of Laboratory Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006, People’s Republic of China
      2. The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510006, People’s Republic of China
      3. Guangzhou University of Chinese Medicine, Guangzhou 510006, People’s Republic of China
      4. Department of Stomatology, Guangzhou Women and Children’s Medical Center, Guangzhou 510623, People’s Republic of China
      5. Present Address: Department of Laboratory Medicine, The Affiliated Hexian Memorial Hospital of Southern Medical University, Guangzhou, People’s Republic of China
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