• Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/046/0063

    • Keywords

       

      Osteoclasts; TNF-$\alpha$; ; RANKL; IL-3

    • Abstract

       

      IL-3, a haematopoiesis regulatory factor, has previously been shown to inhibit both mouse and humanosteoclast differentiation and bone resorption. Here, the role of rat IL-3 on rat osteoclast differentiation was evaluated to address whether the inhibitory action of IL-3 on osteoclastogenesis is conserved in variousspecies. It was observed that IL-3 inhibited rat osteoclast differentiation induced by both TNF-$\alpha$ and receptoractivator of NF-$\kappa$B ligand (RANKL). TNF-$\alpha$ is known to induce bone loss in postmenopausal osteoporoticwomen and it also synergise with many pro-osteoclastogenic cytokines to cause huge pathological bone loss.Importantly, it was found that rat IL-3 inhibits the synergistic action of TNF-$\alpha$ with RANKL and IL-1$\beta$, TGF$\beta$1and TGF-$\beta$3. IL-3 downregulates the TNF-$\alpha$-induced nuclear translocation of NF-$\kappa$B-p65 and c-fos withoutaffecting c-jun. Interestingly, we observed that IL-3 also inhibits osteoclast differentiation in vivo in rats induced by TNF-$\alpha$. All these results suggest that inhibitory action of IL-3 on osteoclastogenesis is conserved invarious species including mice, rats and humans. Thus, our results clearly indicate that IL-3 has therapeuticpotential to treat pathological bone loss in important skeletal diseases.

    • Author Affiliations

       

      VIKRANT PIPRODE1 KANUPRIYA SINGH1 ANIL KUMAR1 SNEHAL R JOSHI1 MOHAN R WANI1

      1. Bone and Cartilage Research Laboratory, National Centre for Cell Science, S. P. Pune University Campus, Ganeshkhind, Pune 411 007, India
    • Dates

       
  • Journal of Biosciences | News

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2021-2022 Indian Academy of Sciences, Bengaluru.