• Target of rapamycin (TOR) gene is vital for whitefly survival and reproduction

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      https://www.ias.ac.in/article/fulltext/jbsc/046/0060

    • Keywords

       

      Bemisia tabaci; RNAi; gene silencing; target of rapamycin; TOR; fecundity

    • Abstract

       

      The whitefly Bemisia tabaci (Gennadius) is a devastating polyphagous pest that causes significant losses inseveral economically important crops worldwide. Although chemical control is the fastest managementstrategy, insecticide resistance development poses a greater constraint in whitefly management. In this study,we used RNA interference (RNAi) technique to understand the role of target of rapamycin (TOR) gene inwhitefly. In insects, TOR plays a crucial role in cell growth, metabolism, proliferation, and reproduction.Although sequence analyses of TOR revealed 95 nucleotides different in TOR gene sequence compared topredicted sequence (GenBank accession no. XM019060193.1), but this led to synthesis of only two differentamino acids at positions 1448 and 1768. Phylogenetic analysis of amino acid sequence revealed that TOR geneof B. tabaci, Acrythosiphum pisum and Nilaparvata lugens were more closely related to each other and wereclustered together. Oral feeding of artificial diet containing dsRNA viz 2.0, 1.0, 0.5, 0.1 lg/ll corresponding toTOR gene to adult whitefly resulted in 42.5% adult mortality, reduced fecundity i.e. 36.57 eggs per female, and62.50% total nymphal mortality in the next generation. The qRT-PCR analysis confirmed the downregulationof TOR gene expression in adult whiteflies upon dstor feeding. These results suggest that TOR gene significantlyregulates the survival and reproduction in whitefly and can be a potential RNAi target for insectmanagement.

    • Author Affiliations

       

      HEENA PURI1 2 VIKAS JINDAL1

      1. Insect Molecular Biology Laboratory, Department of Entomology, Punjab Agricultural University, Ludhiana 141004, India
      2. Present Address: Department of Entomology, University of Nebraska-Lincoln, Lincoln, USA
    • Dates

       
  • Journal of Biosciences | News

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