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      https://www.ias.ac.in/article/fulltext/jbsc/046/0048

    • Keywords

       

      Hydra; human XPA-deficient cells; nucleotide excision repair; UV sensitivity

    • Abstract

       

      Nucleotide excision repair (NER) pathway is a DNA repair mechanism that rectifies a wide spectrum of DNAlesions. Xeroderma pigmentosum group of proteins (XPA through XPG) orchestrate the NER pathway inhumans. We have earlier studied XPA homolog from Hydra (HyXPA) and found it to be similar to humanXPA. Here, we examined if HyXPA can functionally complement human XPA-deficient cells and reduce theirsensitivity to UV radiation. We found that HyXPAwas able to partially rescue XPA-deficient human cells fromUV by its binding to chromatin of UV-irradiated cells. However, HyXPA failed to bind replication protein A(RPA70), a key interacting partner of human XPA in NER pathway. This could be attributed to changes incertain amino acid residues that have occurred during evolution, leading to prevention of some interactionsbetween Hydra and human proteins.

    • Author Affiliations

       

      ALISHA A GALANDE1 2 MASAFUMI SAIJO3 SAROJ S GHASKADBI4 SURENDRA GHASKADBI1

      1. Developmental Biology Group, MACS-Agharkar Research Institute, Pune 411 004, India
      2. Department of Biotechnology, Fergusson College, Pune 411 004, India
      3. Graduate School of Frontier Biosciences, Osaka University, Osaka 565-0871, Japan
      4. Department of Zoology, Savitribai Phule Pune University, Pune 411 007, India
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