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      https://www.ias.ac.in/article/fulltext/jbsc/046/0038

    • Keywords

       

      circRNA Slco3a1; circRNA Wdr33; miRNA; influenza; lung injury

    • Abstract

       

      Circular RNA (circRNA) plays an important role in the regulation of multiple biological processes. However,circRNA profiling and the potential biological role of circRNA in influenza Avirus (IAV)-induced lung injury havenot been investigated. In the present study, circRNA expression profiles in lung tissues frommice with and withoutIAV-induced lung injury were analyzed using high-throughput sequencing, and differentially expressed circRNAswere verified by quantitative PCR. The gene homology of candidate circRNAs was investigated and the expressionof plasma circRNAs frompatients with IAV-induced acute respiratory distress syndrome (ARDS)was detected. Thetarget microRNAs (miRNAs) of circRNAswere predicted.GeneOntology (GO) andKyoto Encyclopedia ofGenesand Genomes (KEGG) analyses were performed. In total, 781 circRNAs were differentially expressed betweenARDS mice and control (467 were up-regulated and 314 were down-regulated).Moreover, the candidate circRNAs(Slco3a1,Nfatc2,Wdr33, andDmd) expression showed the same trendwith the sequencing results. The isoforms ofcircRNA Slco3a1 and Wdr33 were highly conserved between humans and mice. Plasma circRNA Slco3a1 andWdr33 presented differential expression in patients with IAV-induced ARDS compared to control. The circRNAmiRNAinteraction network and GO and KEGG analyses indicated the potential biological role of circRNAs in thedevelopment of IAV-induced lung injury. Taken together, a large number of differentially expressed circRNAs wereidentified in our study. CircRNA Slco3a1 andWdr33 had significantly different expression in specimens from miceand humans, and showed a potential biological role in IAV-induced lung injury by bioinformatics analysis.

    • Author Affiliations

       

      JIAJIA WANG1 YANBING ZHANG2 FENGFENG ZHU3 LILING CHEN4 YAO WEI5 QINGQING ZHU1 JUNHONG JIANG1 JIAN-AN HUANG1 QIANG GUO5 YANG XINJING5

      1. Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou 215000, China
      2. Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215000, China
      3. Department of Emergency and Critical Care Medicine, The Fifth People’s Hospital of Suzhou, Suzhou 215000, China
      4. Suzhou Center for Disease Control and Prevention, Suzhou 215004, China
      5. Department of Emergency and Critical Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou 215000, China
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