Currently, we are at the threshold of the ‘post-antibiotic era’ due to the global emergence of antimicrobialresistance (AMR), and hence there is a dire need to discover new antibiotics. Ribosomally synthesized andpost-translationally modified peptides (RiPPs) are a diverse class of natural products (NPs), some of which areunder clinical trials for their antimicrobial potential. Thiopeptides are structurally one of the most complexclasses of RiPPs due to numerous post-translational modifications (PTMs), with [4?2] cycloaddition being thecore PTM and are active against several gram-positive pathogens. Genome mining coupled with experimentalwork can harness the unexplored ‘cryptic’ gene clusters while minimizing the rate of the rediscovery of knownmetabolites and expand the molecular diversity of NPs with medicinal potential. Employing the genomemining approach using a series of freely available bioinformatics tools, we have identified eight novel putativethiopeptide encoding biosynthetic gene clusters (BGCs) from different bacterial genomes, most of whichbelong to the class Actinobacteria. Our results provide confidence in the newly identified BGCs, to proceedwith wet-bench experiments and discover novel thiopeptide(s).
Volume 46, 2021
Continuous Article Publishing mode
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