• Fulltext


        Click here to view fulltext PDF

      Permanent link:

    • Keywords


      Phytic acid; myo-inositol-1,3,4 trisphosphate-5/6 kinase; ITPK; in silico analysis; RNAi silencing; inorganic phosphorus; micronutrients; rice

    • Abstract


      Phytic acid or Myo-inositol hexakisphosphate is an essential compound for the rice plants. It remains in theform of phytate, a mixed salt of different mineral cations, in the seeds. The phytate breaks down duringgermination and provides the inorganic phosphorus and mineral ions to the seedlings. However, humans do notget the benefit of those essential ions from rice consumption due to the absence of phytase in the gut. Weenvisaged down-regulating ITPK, the gene behind the phytic acid biosynthesis so that its low amount wouldfacilitate a greater amount of free mineral ions in the endosperm. Since there are six homologues of rice ITPK,we studied their expression in seeds. Additionally, we undertook an in-silico analysis of the homologousproteins. Considering the results, we selected ITPK-2 for its RNAi-mediated embryo-specific down-regulationto obtain the low phytate rice. We obtained a 37% reduction of phytic acid content accompanied by a nearlythree-fold enhancement of inorganic phosphorus in the transgenic seeds. Additionally, the iron and zinc contentincreased in polished rice grains compared to the wild type. The results also showed that reduced phytic acidcontent did not affect the germination potential and seedling growth of the transgenic rice.

    • Author Affiliations

    • Dates

    • Supplementary Material

  • Journal of Biosciences | News

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2021-2022 Indian Academy of Sciences, Bengaluru.