• Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/046/0029

    • Keywords

       

      Achaea janata; altered expression; aminopeptidase N; Bacillus thuringiensis; cry toxin; midgut

    • Abstract

       

      The midgut of lepidopteran larvae is a multifunctional tissue that performs roles in digestion, absorption,immunity, transmission of pathogens and interaction with ingested various molecules. The proteins localized atthe inner apical brush border membrane are primarily digestive proteases, but some of them, likeaminopeptidase N, alkaline phosphatase, cadherins, ABC transporter C2, etc., interact with Crystal (Cry) toxinsproduced by Bacillus thuringiensis (Bt). In the present study, aminopeptidase N (APN) was characterized asCry-toxin-interacting protein in the larval midgut of castor semilooper, Achaea janata. Transcriptomic andproteomic analyses revealed the presence of multiple isoforms of APNs (APN1, 2, 4, 6 and 9) which have lessthan 40% sequence similarity but show the presence of characteristic ‘GAMENEG’ and zinc-binding motifs.Feeding a sublethal dose of Cry toxin caused differential expression of various APN isoform. Further, 6thgenerationCry-toxin-exposed larvae showed reduced expression of APN2. This report suggests that A. janatalarvae exploit altered expression of APNs to overcome the deleterious effects of Cry toxicity, which mightfacilitate toxin tolerance in the long run.

    • Author Affiliations

       

      VINOD K CHAUHAN1 2 NARENDER K DHANIA1 3 VADTHYA LOKYA4 BHOOPAL BHUVANACHANDRA5 KOLLIPARA PADMASREE4 APARNA DUTTA-GUPTA1

      1. Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad 500 046, India
      2. Department of Zoology, Visva-Bharati University, Shantiniketan 731 235, India
      3. Department of Human Genetics and Molecular Medicine, School of Health Sciences, Central University of Punjab, Bathinda 151 001, India
      4. Departments of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, Hyderabad 500 046, India
      5. Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500 046, India
    • Dates

       
    • Supplementary Material

       
  • Journal of Biosciences | News

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2021-2022 Indian Academy of Sciences, Bengaluru.