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      https://www.ias.ac.in/article/fulltext/jbsc/046/0026

    • Keywords

       

      Pseudomonas aeruginosa; multidrug resistance; imipenem; silver nanoparticle; conjugation; antibacterial

    • Abstract

       

      Due to the broad-spectrum of antibiotic resistance, herein we investigated the possibility of using imipenemconjugatedsilver nanoparticles (IMP-AgNPs) against multidrug-resistant isolates of Pseudomonas aeruginosa.For this purpose, 200 clinical isolates were tested against different antibiotics to determine the antimicrobialsusceptibility. To identify blaVIM and blaIMP resistance genes, PCR was used. The synthesized AgNPs andconjugants were characterized using UV-vis spectroscopy, XRD, SEM, TEM, DLS, and FTIR. The stability,drug release kinetics, cytotoxicity, hemolytic and apoptotic effects of NPs were also investigated. MIC of theimipenem, AgNPs, and conjugants were evaluated versus P. aeruginosa isolates. Finally, the effects of theIMP-AgNPs to heal burn wounds in rats was evaluated. According to the results, about 68% of isolates showedresistance to imipenem (MIC >= 64 lg/ml to >=512 lg/ml). Analytical results verified the synthesis of AgNPsand IMP-AgNPs. A Dose-dependent decrease happened in terms of the MIC values of IMP-AgNPs were alsoaffected by the existence of resistant genes. Low cytotoxic was observed regarding AgNPs which lead toapoptosis. The histopathological results showed a considerable epithelization in treated groups with IMPAgNPs.Accordingly, IMP-AgNPs can be considered as a powerful antibacterial agent to treat the infectionscaused by multidrug-resistant P. aeruginosa.

    • Author Affiliations

       

      MAHSA SHAHBANDEH1 ANOOSH EGHDAMI2 MEHRDAD MOOSAZADEH MOGHADDAM3 MOHAMMADREZA JALALI NADOUSHAN4 ALI SALIMI5 MAHDI FASIHI-RAMANDI6 SABER MOHAMMADI7 MEHRNAZ MIRZAEI8 REZA MIRNEJAD6

      1. Young Researchers and Elite Club, Saveh Branch, Islamic Azad University, Saveh, Iran
      2. Department of Biochemistry, Medical Faculty Member, Saveh Branch, Islamic Azad University, Saveh, Iran
      3. Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
      4. Department of Pathology, Shahed University, Tehran, Iran
      5. Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
      6. Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
      7. Young Researchers and Elite Club, Islamic Azad University, Tehran, Iran
      8. Department of Microbiology, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
    • Dates

       
  • Journal of Biosciences | News

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