• Circ-HIPK3 regulates YAP1 expression by sponging miR-381-3p to promote oral squamous cell carcinoma development

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      https://www.ias.ac.in/article/fulltext/jbsc/046/0020

    • Keywords

       

      Circ-HIPK3; miR-381-3p; YAP1; OSCC; progression

    • Abstract

       

      Circular RNAs (circRNAs) have been reported to play important roles in human cancers. Circular RNAhomeodomain interacting protein kinase 3 (Circ-HIPK3) was investigated to be involved in tumorigenesis.However, the functions of circ-HIPK3 in oral squamous cell carcinoma (OSCC) remain vague. The expressionof circ-HIPK3, microRNA (miR)-381-3p and Yes-associated protein1 (YAP1) was detected by qRT-PCR orwestern blot. Cell proliferation, apoptosis, invasion and migration were measured by MTT assay, flowcytometry, or transwell assay. The dual-luciferase reporter assay was employed to test the target correlationsmiR-381-3p and circ-HIPK3 or YAP1. Murine xenograft model was established to conduct in vivo assay. Circ-HIPK3 was elevated in OSCC tissues and cell lines, and decrease of circ-HIPK3 suppressed OSCC cellproliferation, invasion, migration and induced apoptosis in vitro as well as inhibited tumor growth in vivo.Rescue assay indicated circ-HIPK3 silence mediated OSCC progression inhibition by sponging miR-381-3p,which was a target of circ-HIPK3. Furthermore, miR-381-3p directly interacted with YAP1 and miR-381-3pinhibition could attenuate YAP1 deletion-induced suppression on cell malignant biological behavior in OSCC.Meanwhile, co-expression analysis showed circ-HIPK3 could regulate YAP1 expression by competing formiR-381-3p. Circ-HIPK3 contributed to OSCC growth and development through regulating YAP1 expressionby sponging miR-381-3p, indicating a promising therapeutic strategy for OSCC.

    • Author Affiliations

       

      LEI BI1 CHUNGUANG ZHANG1 YUAN YAO1 ZHAO HE2

      1. Department of Stomatology, North China University of Science and Technology Affiliated Hospital, Tangshan 063000, China
      2. Department of Anesthesiology, The Second Hospital of Tangshan, Tangshan 063000, China
    • Dates

       
  • Journal of Biosciences | News

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