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    • Keywords


      CDK8; H9c2; hypoxia; miR-9; Wnt/beta-catenin

    • Abstract


      Hypoxia plays an important role in many heart diseases. MicroRNA-9 (miR-9) has been reported to beinvolved in hypoxia-induced cell proliferation, injury and apoptosis in cardiomyocytes. However, the underlyingmechanism still remains poorly understood. The expression levels of miR-9 and cyclin-dependent kinase8 (CDK8) were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The relative proteinexpression was measured by Western blot. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide(MTT), lactate dehydrogenase (LDH) measurement, flow cytometry assays were conducted to detect cellproliferation, the release of LDH and cell apoptosis, respectively. The potential relationship between miR-9 andCDK8 was predicted by online database, and confirmed by dual-luciferase reporter assay. We found that miR-9was increased, while CDK8 was decreased in hypoxia-treated H9c2 cells. miR-9 down-regulation or CDK8up-regulation promoted cell proliferation, while repressed cell damage and apoptosis in hypoxia-induced H9c2cells. Moreover, CDK8 was identified to be target of miR-9, and CDK8 knockdown could reverse the effects ofmiR-9 inhibitor on cell proliferation, damage and apoptosis in hypoxia-treated H9c2 cells. Besides, miR-9could regulate the Wnt/b-catenin pathway by targeting CDK8 in hypoxic-induced H9c2 cells. In conclusion,miR-9 repressed cell proliferation and promoted cell damage and apoptosis by binding to CDK8 through theWnt/beta-catenin pathway in hypoxic-induced H9c2 cells, which provided a new direction for further studying thetreatment of hypoxia-aroused heart diseases.

    • Author Affiliations



      1. Department of Orthopedics, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan, People’s Republic of China
      2. Xiangya School of Medicine, Central South University, Changsha 410011, Hunan, People’s Republic of China
      3. Department of Geriatrics, The Second Xiangya Hospital of Central South University, No.139, Ren Min Road, Changsha 410011, Hunan, People’s Republic of China
    • Dates

  • Journal of Biosciences | News

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      Posted on July 25, 2019

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