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      https://www.ias.ac.in/article/fulltext/jbsc/046/0008

    • Keywords

       

      3T3-L1 adipocytes; Adipogenesis; Apocynin; Insulin signaling cascade; Type 2 diabetes

    • Abstract

       

      Adipogenesis is a cascade of processes that entail the differentiation of fibroblasts into mature adipocytes,which results in the accumulation of triglycerides in the adipose cells due to high dietary supplements. Thisphysiological condition increases the risk of type 2 diabetes. Apocynin (4-hydroxy-3-methoxyacetophenone),an organic compound from the root extracts of the medicinal herb Picrorhiza kurroa, has been used in variousexperimental studies. The current study focuses on deciphering the cellular and molecular mechanismsinterlinking obesity and diabetes by validating the various key targets involved in insulin signaling andadipogenesis. Apocynin exhibited enhanced glucose uptake and decreased lipid accumulation in the adipocytes.Furthermore, the expression of molecular markers involved in the insulin signaling pathway, such asIRTK, IRS-1, PI3K, GLUT-4, and the adipogenic pathway, such as PPAR-alpha, adiponectin, C/EBP-alpha andSREBP1C, by qPCR supported our hypothesis largely. Apocynin mimicked insulin in the insulin-signalingpathway by showing equivalent gene expression. It ameliorated adipogenesis by downregulating the keymarkers in the adipogenic pathway. Corroborating the hypothesis that Apocynin is antihyperlipidemic innature, it reduced the expression of PPAR-alpha and adiponectin. These results substantiate that Apocynin exertsanti-diabetic and anti-adipogenic effects by regulating resistin and antioxidant enzyme levels in vitro.

    • Author Affiliations

       

      SAI BHARADWAJA1 PRAVEEN KUMAR ISSAC1 JOCELYN CLETA1 RAKESH JEGANATHAN1 SRI SNEHAA CHANDRAKUMAR2 SUJATHA SUNDARESAN1

      1. Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Kancheepuram District, Tamil Nadu 603 203, India
      2. Department of Biotechnology, Anna University, BIT Campus, Tiruchirappalli, Tamil Nadu 620 024, India
    • Dates

       
  • Journal of Biosciences | News

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