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      https://www.ias.ac.in/article/fulltext/jbsc/046/0004

    • Keywords

       

      Acute myeloid leukemia; CELF2; LncRAN SNHG16; PI3K/AKT signaling; PTEN

    • Abstract

       

      The silence of lncRNA small nucleolar RNA host gene 16 (SNHG16) suppressed acute lymphoblastic leukemia(ALL) cell proliferation and migration, whereas its role in acute myeloid leukemia (AML) still lacksclarity. This study showed that SNHG16 was upregulated in AML patients and cells. And SNHG16 overexpressionremarkably enhanced the proliferation and migration capacities of HL60 and AML-193 cells, whileSNHG16 knockdown acted the opposite way. Subsequently, we revealed that SNHG16 directly bound toCELF2 (CUGBP Elav-like family member 2) protein, and caused CELF2 mRNA unstably and proteinsreducing. CELF2 was decreased both in AML patients and cells. CELF2 overexpression or interferenceweakened the effect of overexpressing or silencing SNHG16 on proliferation and migration. Moreover, thetransfection of pcDNA-CELF2 elevated PTEN (phosphatase and tensin homolog) activity and hindered thephosphoinositide 3-kinase (PI3K)/AKT signaling. And SNHG16 reduced PTEN activity and promoted thePI3K/AKT pathway activation by restraining CELF2. Furthermore, GDC-0941 (a specific inhibitor of thePI3K/AKT pathway) impeded the effect of SNHG16 increase, and bpV(pic) (a specific PTEN inhibitor)declined the effect of SNHG16 decrease on cell proliferation and migration. Taken together, the present studyindicated that SNHG16 promoted proliferation and migration of AML cells via PTEN/PI3K/AKT axis throughsuppressing CELF2 protein.

    • Author Affiliations

       

      MIN SHI1 RUILI YANG2 JING LIN3 QI WEI1 LEI CHEN1 WEIFENG GONG4 YANG LI5 XIAOBO GUO3

      1. School of Medicine, Xi’an Peihua University, 888 Changning Street, Xi’an 710125, China
      2. Clinical Laboratory, Xi’an Chang’an Hospital, 17 Wenjing Road, Xi’an 710016, China
      3. Department of Hematology, Xi’an Central Hospital, 161 Xiwu Road, Xi’an 710003, China
      4. Department of Blood Transfusion, Xi’an Central Hospital, 161 Xiwu Road, Xi’an 710003, China
      5. Clinical Laboratory, Xi’an Central Hospital, 161 Xiwu Road, Xi’an 710003, China
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