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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/045/0145

    • Keywords

       

      H19; long non-coding RNAs; oral cancer; oral squamous cell carcinoma; TCGA analysis

    • Abstract

       

      Long non-coding RNAs (lncRNAs) are a group of non-protein-coding RNAs which are longer than 200nucleotides. LncRNAs play important roles in epigenetic modification, transcription and post-transcriptionalregulation, maintenance of normal tissue development and differentiation. LncRNA could serve as a biomarkerfor diagnosis and prognosis as well as a molecular target for therapy in oral squamous cell carcinoma (OSCC).Therefore, we have determined the expression profile of 5-lncRNAs namely UCA1, TUG1, HOTAIR,MALAT1, and H19 by quantitative real-time PCR in tumor tissues and adjacent normal tissue of 32 OSCCpatients. To determine the expression, methylation status and genomic alterations in lncRNAs across pancancer,TCGA datasets were analyzed by UALCAN, MEXPRESS and cBioPortal database. Then, we determinedthe association between lncRNA expression and clinicopathological attributes of patients by Spearman’srank test. Expression of UCA1 and TUG1 genes was up-regulated in 54.83% and 53.12% OSCC tumors,respectively. Importantly, expression of MALAT1 and H19 was down-regulated in tumor tissues of 62.5% and81.25% respectively of OSCC patients. Except for MALAT1, our experimental data showed concordance withthe TCGA analysis. Expression of HOTAIR in OSCC tumors was positively correlated with tumor volume,whereas MALAT1 and H19 negatively correlated with the smoking status of patients.

    • Author Affiliations

       

      SUPRIYA VISHWAKARMA1 RITU PANDEY1 RENU SINGH2 RAGINI GOTHALWAL3 ASHOK KUMAR1

      1. Department of Biochemistry, All India Institute of Medical Sciences (AIIMS), Saket Nagar, Bhopal 462 020, India
      2. Jawaharlal Nehru Cancer Hospital and Research Centre (JNCHRC), Idgah Hills, Bhopal, India
      3. Department of Biotechnology, Barkatullah University, Hoshangabad Road, Bhopal, India

    • Dates

       
      Published
      23 November 2020

    • Supplementary Material

       

  • Journal of Biosciences | News

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    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

      Posted on April 12, 2023
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