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https://www.ias.ac.in/article/fulltext/jbsc/045/0127

Cell viability; DSCC1; GSK-3$\beta$/$\beta$-catenin; migration and invasion

The objective of this study is to assess the prognostic and functional role of DSCC1 in breast carcinoma, aswell as the potential mechanism. Based upon the TCGA data, the expression pattern and prognostic value ofDSCC1 in breast carcinoma was evaluated. The mRNA and protein levels of molecules were determined usingqRT-PCR and Western blot. In vitro functional role of DSCC1 in tumor cells was determined using cellcounting kit 8, clone formation, and Transwell assays. Gene set enrichment analysis (GSEA) was conducted todetermine DSCC1 related gene sets, which are further confirmed by Western blot. The results showed thatDSCC1 is overexpressed in breast carcinoma tissues and its high expression was linked to shorter overallsurvival. Overexpression of DSCC1 facilitated the proliferation, invasion and migration of breast carcinomacells, while knockdown of DSCC1 showed opposite outcomes. GSEA showed that high DSCC1 expressionhad a positive correlation with p53, and Wnt signaling-related molecules. Western blot showed that silencingDSCC1 increased the levels of p53 and p-$\beta$-catenin, whereas decreased p-GSK-3$\beta$ and cyclin D1 expression.These observations illustrate that DSCC1 emerges a well value on the diagnosis and prognosis of breastcarcinoma, and facilitates the progression of breast carcinoma partly by activating Wnt/$\beta$-catenin signaling andinhibiting p53.

GUANGCHAO JIN¹ WENSHENG WANG² PENG CHENG³ YUNQI TIAN⁴ LUXIAO ZHANG¹ HU NIU¹

1. Department of Breast and Thyroid Surgery, Jinan Central Hospital Affiliated to Shandong First Medical University, Jinan 250013, Shandong, China
2. Department of Emergency Medicine, Xintai People’s Hospital, Xintai 271200, Shandong, China
3. Department of General Surgery, No.2 People’s Hospital of Xintai, Xintai 271219, Shandong, China
4. Department of Gastroenterology Department 3, Jinan Central Hospital Affiliated to Shandong First Medical University, Jinan 250013, Shandong, China

Published

13 October 2020