• Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/045/0119

    • Keywords

       

      CaMV35S promoter; constitutive promoters; synthetic promoters; genetic engineering; RNA pol II promoters; RNA pol III promoters; U3 promoter; U6 promoter

    • Abstract

       

      Genetically engineered plants have varied applications in agriculture for enhancing the values of food and feed.Genetic engineering aims to introduce selected genetic regions with desirable traits into target plants for bothspatial and temporal expressions. Promoters are the key elements responsible for regulating gene expressionsby modulating the transcription factors (TFs) through recognition of RNA polymerases. Based on theirrecognition and expression, RNA polymerases were categorized into RNA pol II and pol III promoters.Promoter activity and specificity are the two prime parameters in regulating the transgene expression. Since theuse of constitutive promoters like Cauliflower mosaic virus (CaMV) 35S may lead to adverse effects on nontargetorganisms or ecosystem, inducible/tissue specific promoters and/or the RNA pol III promoters providemyriad opportunities for gene expressions with controlled regulation and with minimum adverse effects.Besides their role in transgene expression, their influence in synthetic biology and genome editing are alsodiscussed. This review provides an update on the importance, current prospects, and insight into the advantagesand disadvantages of promoters reported thus far would help to utilize them in the endeavour to developnutritionally and agronomically improved transgenic crops for commercialization.

    • Author Affiliations

       

      DIVYA KUMMARI1 2 SUDHAKAR REDDY PALAKOLANU1 P B KAVI KISHOR3 POOJA BHATNAGAR-MATHUR1 PRASANTH SINGAM2 VINCENT VADEZ1 KIRAN K SHARMA1

      1. International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad 502 324, India
      2. Department of Genetics, Osmania University, Hyderabad 500 007, India
      3. Department of Biotechnology, Vignan’s Foundation for Science, Technology and Research Deemed to be University, Vadlamudi, Guntur 522 213, India
    • Dates

       
  • Journal of Biosciences | News

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2021-2022 Indian Academy of Sciences, Bengaluru.