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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/045/0089

    • Keywords

       

      Annona muricata; fibrosarcoma; MMP-2 and MMP-9; RECK; TIMP-2

    • Abstract

       

      Up-regulation of MMP-2 and MMP-9 plays a significant role in promoting cancer progression by degradingthe components of the extracellular matrix, thereby enhancing the migration of tumor cells. Although the antiproliferativeand apoptotic effect of Annona muricata is well established, its effect on MMP-2 and MMP-9, amajor target in several types of cancers, has not been studied. Powdered samples of various parts of A.muricata like fruit, stem, seed, and twig extracted using aqueous methanol showed significant dose-dependentinhibition of MMP-2 and MMP-9 in a highly metastatic fibrosarcoma cell line, HT1080. Additionally, theseextracts also up-regulated the expression of several endogenous inhibitors of MMP-2 and MMP-9 likeREversion-inducing Cysteine-rich protein with Kazal motifs (RECK) and Tissue Inhibitor of Metalloproteinase-2 (TIMP-2). Furthermore, primary cells developed from tumor tissues obtained from patients notexposed to chemotherapy, also exhibited similar results. Remarkably, the inhibition of MMP-2 and MMP-9observed was tumor specific, with the A. muricata fruit extract showing only 2% inhibition in cells obtainedfrom normal tissues, when compared to 60% inhibition observed in cells obtained from tumor samples. Thepresent study elucidates a novel mechanism by which A. muricata extracts selectively exhibit their anti-canceractivity in tumor cells by down-regulating MMP-2 and MMP-9 that are important biomarkers in cancer.

    • Author Affiliations

       

      G DRISHYA1 JYOTSNA NAMBIAR1 SANU K SHAJI1 MURALIDHARAN VANUOPADATH1 A ACHUTHAN1 ABISHEK KUMAR1 ASHNA ALIAS1 AYESHA SHERIF1 CATHERINE JOSEPH1 P DIVYA1 DAMU SUNIL KUMAR1 CHINCHU BOSE1 SOBHA VIJAYAN NAIR1 S SUDARSLAL1 GEETHA B KUMAR1 S LAKSHMI2 BIPIN G NAIR1

      1. Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Clappana P.O, Kollam 690 525, India
      2. Laboratory of Molecular Medicine, Division of Cancer Research, Regional Cancer Centre, Thiruvananthapuram 695 011, India
    • Dates

       
  • Journal of Biosciences | News

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