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      https://www.ias.ac.in/article/fulltext/jbsc/045/0081

    • Keywords

       

      Fibrin scaffold; mesenchymal stem cells; Nanog; OCT4; SOX2; stemness

    • Abstract

       

      In regenerative medicine, MSCs need to be pluripotent for better results. In this study, the effect of fibrinscaffold on expression of stemness genes was examined. Adipose-derived MSCs were cultured in tissue cultureplates (2D) and 3-dimensional (3D) fibrin scaffolds. The effect of fibrin scaffold on proliferation of adiposederivedMSCs was evaluated by MTT assay. The expression of stemness genes (OCT4 and SOX2) wereevaluated by qRT-PCR, and flow cytometry was done for Nanog protein level. Cultured MSCs on fibrinscaffold were able to proliferate according to data obtained by MTT assay. Expression of OCT4 and SOX2 hada significant increase in cells were cultured in 3D condition compared to 2D condition (P less than 0.05). Also,increased expression of Nanog protein in 3D culture was observed (P less than 0.05). OCT4 and SOX2 in 3Dcondition increased two-fold and three-fold respectively in 2D and 3D conditions. Moreover, expression ofNanog increased 30% more than in 2D condition. Evaluation of important pluripotency regulators such asOCT4, SOX2, and Nanog showed that fibrin scaffolds are useful instruments to maintain stemness of MSCs,which is essential in field of stem cell therapy and regenerative medicine.

    • Author Affiliations

       

      AREZOO MOUSAVIFARD1 ELAHE POURAZIZI NAJAFABADI2 MAHSHID AKHAVAN RAHNAMA3 AZADEH ANBARLOU4 5 AMIR ATASHI6

      1. Department of Tissue Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran
      2. Department of Biochemistry, Najafabad Branch, Islamic Azad University, Najafabad, Iran
      3. Department of Hematology and Cell Therapy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
      4. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
      5. Present Address: Department of Laboratory Medicine, Institute of Biomedicine, Sahlgrenska Academy at University of Gothenburg, Go¨teborg, Sweden
      6. Stem Cell and Tissue Engineering Research Center, Shahroud University of Medical Sciences, Shahroud, Iran
    • Dates

       
  • Journal of Biosciences | News

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