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      https://www.ias.ac.in/article/fulltext/jbsc/045/0080

    • Keywords

       

      Cervical cancer; chemotherapy; CRISPR knockdown; HPV-positive and HPV-negative

    • Abstract

       

      Cervical cancer is the fourth most common cause of mortality in women worldwide. In this study weinvestigated the effect of a tumour suppressor microRNA miR-214 in modulating the cell death againstchemotherapeutic drugs like Doxorubicin, Cisplatin and Paclitaxel. CRISPR-facilitated knockdown andplasmid-based overexpression of miR-214 was performed in cervical cancer cell lines HeLa, C33A and CaSki.It was observed that knocking out miR-214 resulted in reduced apoptosis and cell migration upon drugtreatments; while overexpression of miR-214 resulted in marginal increase in apoptosis and cell migrationwhen treated with drugs. However, miR-214 had very little effect on production of reactive oxygen species.Our results also indicate that Doxorubicin was least effective and Paclitaxel most effective in inducing celldeath. A combination of miR-214 overexpression and Paclitaxel treatment was found to be most effective ininducing cell death in cervical cancer cells. Analysis of cell cycle phases followed by apoptotic markers alsoshowed that miR-214 overexpression along with Paclitaxel treatment caused an increase in PARP and declineof PI-3 kinase/Akt levels. Therefore, miR-214 levels determine the fate of the cancer cell duringchemotherapeutic treatment.

    • Author Affiliations

       

      PRAKRITI SEN1 SAYAM GHOSAL1 RUDRANIL HAZRA1 RIMJHIM MOHANTY1 SOLOMON AREGA1 BIKASH SAHU2 NILADRI GANGULY1

      1. Cancer Biology Lab, School of Biotechnology, Kalinga Institute of Industrial Technology, Bhubaneswar 751 024, India
      2. Infection Biology Lab, School of Biotechnology, Kalinga Institute of Industrial Technology, Bhubaneswar 751 024, India
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    • Supplementary Material

       
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