-
- Home
- Journals
- Journal of Biosciences
- Volume 45
- All articles
-
Mutations in SARS-CoV-2 viral RNA identified in Eastern India: Possible implications for the ongoing outbreak in India and impact on viral structure and host susceptibility
ARINDAM MAITRA MAMTA CHAWLA SARKAR HARSHA RAHEJA NIDHAN K BISWAS SOHINI CHAKRABORTI ANIMESH KUMAR SINGH SHEKHAR GHOSH SUMANTA SARKAR SUBRATA PATRA RAJIV KUMAR MONDAL TRINATH GHOSH ANANYA CHATTERJEE HASINA BANU AGNIVA MAJUMDAR SREEDHAR CHINNASWAMY NARAYANASWAMY SRINIVASAN SHANTA DUTTA SAUMITRA DAS
Article Volume 45 Published: 4 June 2020 Article ID 0076
-
-
Fulltext
Click here to view fulltext PDF
Permanent link:
https://www.ias.ac.in/article/fulltext/jbsc/045/0076 -
Keywords
Host miRNA ;molecular phylogeny ;protein structure ;SARS-CoV-2 ;viral RNA sequence -
Abstract
Direct massively parallel sequencing of SARS-CoV-2 genome was undertaken from nasopharyngeal andoropharyngeal swab samples of infected individuals in Eastern India. Seven of the isolates belonged to the A2aclade, while one belonged to the B4 clade. Specific mutations, characteristic of the A2a clade, were alsodetected, which included the P323L in RNA-dependent RNA polymerase and D614G in the Spike glycoprotein.Further, our data revealed emergence of novel subclones harbouring nonsynonymous mutations, viz.G1124V in Spike (S) protein, R203K, and G204R in the nucleocapsid (N) protein. The N protein mutationsreside in the SR-rich region involved in viral capsid formation and the S protein mutation is in the S2 domain,which is involved in triggering viral fusion with the host cell membrane. Interesting correlation was observedbetween these mutations and travel or contact history of COVID-19 positive cases. Consequent alterations ofmiRNA binding and structure were also predicted for these mutations. More importantly, the possibleimplications of mutation D614G (in SD domain) and G1124V (in S2 subunit) on the structural stability of Sprotein have also been discussed. Results report for the first time a bird’s eye view on the accumulation ofmutations in SARS-CoV-2 genome in Eastern India.
-
Author Affiliations
ARINDAM MAITRA1 MAMTA CHAWLA SARKAR2 HARSHA RAHEJA3 NIDHAN K BISWAS1 SOHINI CHAKRABORTI4 ANIMESH KUMAR SINGH1 SHEKHAR GHOSH1 SUMANTA SARKAR1 SUBRATA PATRA1 RAJIV KUMAR MONDAL1 TRINATH GHOSH1 ANANYA CHATTERJEE2 HASINA BANU2 AGNIVA MAJUMDAR2 SREEDHAR CHINNASWAMY1 NARAYANASWAMY SRINIVASAN4 SHANTA DUTTA2 SAUMITRA DAS1 3
- National Institute of Biomedical Genomics, PO: NSS, Kalyani 741 251, India
- ICMR-National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road, Scheme XM, Beleghata, Kolkata 700 010, India
- Department of Microbiology and Cell Biology, Indian Institute of Science, Bengaluru 560 012, India
- Molecular Biophysics Unit, Indian Institute of Science, Bengaluru 560 012, India
-
Dates
- Published
- 4 June 2020
-
Supplementary Material
-
-
Journal of Biosciences
Volume 48, 2023
All articles
Continuous Article Publishing mode
-
Journal of Biosciences | News
-
To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".
Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.
Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.
Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx
Posted on April 12, 2023< -
Editorial Note on Continuous Article Publication
Posted on July 25, 2019Click here for Editorial Note on CAP Mode
Forthcoming Special issue.
-