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https://www.ias.ac.in/article/fulltext/jbsc/045/0071

Halofuginone; TGF-& beta; 1; EMT; eIF2& alpha; ; IBD

Halofuginone (HF) is an extract from the widely used traditional Chinese medicine (TCM) Dichroa febrifugathat facilitates the recovery of wounds and attenuates hepatic fibrosis. However, the role of HF in theepithelial-mesenchymal transition (EMT) of IPEC-J2 cells remains unclear. The current study explored theanti-EMT effect of HF in IPEC-J2 cells and illustrates its molecular mechanism. Transforming growth factorb1 (TGF-beta-1), as a recognized profibrogenic cytokine, decreased the level of the epithelial marker E-cadherinand increased the level of the mesenchymal markers, such as N-cadherin, fibronectin (FN), vimentin (Vim),and a-smooth muscle actin (a-SMA), in IPEC-J2 cells depending on the exposure time and dose. HF markedlyprevented the EMT induced by TGF-beta-1. Dissection of the mechanism revealed that HF inhibited IPEC-J2 cellEMT via modulating the phosphorylation of SMAD2/3 and the SMAD2/3-SMAD4 complexnuclear translocation. Furthermore, HF could promote the phosphorylation of eukaryotic translation initiationfactor-2-alpha (eIF2-alpha), which modulates the SMAD signaling pathway. These results suggested that HF inhibitsTGF-beta-1-induced EMT in IPEC-J2 cells through the eIF2a/SMAD signaling pathway. Our findings suggest thatHF can serve as a potential anti-EMT agent in intestinal fibrosis therapy.

MINGYUAN DUAN¹ XIAOBING WEI¹ ZHE CHENG¹ DUNJIANG LIU² HANNA FOTINA³ XIAOJING XIA¹ JIANHE HU¹

1. College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China
2. Institute for Metabolic and Neuropsychiatric Disorders, Binzhou Medical University, Binzhou, China
3. Sumy National Agrarian University, Sumy, Ukraine

Published

21 May 2020