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      https://www.ias.ac.in/article/fulltext/jbsc/045/0067

    • Keywords

       

      Polarity protein; Lgl2; influenza A virus; nucleoprotein; nuclear export

    • Abstract

       

      In this study, the regulatory effect of the overexpression of polarity protein Lgl2 on the nuclear export ofinfluenza A virus nucleoprotein in infected cells was investigated. A stable Tet-Off inducible MDCK cell lineexpressing a fusion protein comprising Lgl2 and an enhanced yellow fluorescent protein were used. TCID50analysis and neuraminidase activity analysis revealed that replication of influenza Avirus was inhibited in Lgl2overexpressing cells. By immunofluorescence microscopical observation at different time point post virusinfection, a retention of NP in cellular nucleus was found in Lgl2 overexpressing cells. Compared with normalMDCK cells, change in claudin-1 distribution between cell contacts caused by Lgl2 overexpression impairedthe barrier function of tight junction. These results suggest that changes in cell polarity induced by Lgl2overexpressing will affect virus NP transportation.

    • Author Affiliations

       

      JING LIU1 HAIYING WANG2 MENGDAN FANG1 XUEXIN CHEN1 XIAOBO ZENG1

      1. School of Pharmaceutical Science, Wuhan University, 185 Donghu Road, Wuhan 430071, Hubei, China
      2. College of Life Science, South-Center University for Nationalities, Wuhan 430074, Hubei, China
    • Dates

       
  • Journal of Biosciences | News

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