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      https://www.ias.ac.in/article/fulltext/jbsc/045/0066

    • Keywords

       

      Ovarian cancer; natural killer cells; miR-140-3p; MAPK1; cytotoxicity

    • Abstract

       

      Natural killer (NK) cells have pivotal role in immunotherapy of human ovarian cancer (OC). AlthoughmicroRNAs (miRNAs) participate in dysfunction of NK cells, how and whether miR-140-3p regulates cytotoxicityof NK cells in OC are uncertain. miR-140-3p and mitogen activated protein kinase 1 (MAPK1)abundances were examined via quantitative real-time polymerase chain reaction or western blot. Tumornecrosis factor-a (TNF-alpha) and interferon-c (IFN-gamma) abundances were examined via enzyme linkedimmunosorbent assay. NK cytotoxicity to OC was evaluated via lactate dehydrogenase release. The relevanceof miR-140-3p and MAPK1 was proved via luciferase activity analysis. Murine xenograft experiment wasapplied to assess the function of miR-140-3p on NK cytotoxicity. miR-140-3p was elevated and MAPK1 wasdeclined in NK cells from OC patients, while the levels were reversed after treatment of interleukin-2 (IL-2).MiR-140-3p addition mitigated IFN-gamma and TNF-alpha production induced via IL-2 as well as NK-92 cytotoxicityto OC cells. Additionally, MAPK1 was negatively regulated via miR-140-3p and ablated the influence of miR-140-3p on cytotoxicity, cytokines levels. Besides, miR-140-3p enrichment facilitated tumor growth via suppressingfunction of NK cells in a xenograft model. miR-140-3p suppressed NK cytotoxicity to OC cells viamediating MAPK1, indicating a new avenue of ameliorating NK cells function for OC treatment.

    • Author Affiliations

       

      JIAYING WANG1 MIAOHUA ZHU1 XIAOMING ZHOU1 TINGTING WANG1 YANNI XI2 ZHANG JING1 WENJIN XI3

      1. Department of Gynecology and Obstetrics, The affiliated Hospital of Medical School of Ningbo University, Ningbo 31500, Zhejiang, China
      2. Department of Gynecology and Obstetrics, Yulin No.2 Hospital, Yulin 719000, Shaanxi, China
      3. Department of Immunology, The Fourth Military Medical University, Xi’an 710032, Shaanxi, China
    • Dates

       
  • Journal of Biosciences | News

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