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      https://www.ias.ac.in/article/fulltext/jbsc/045/0062

    • Keywords

       

      Arachis hypogaea L.; microRNAs; nodulation; symbiosis

    • Abstract

       

      microRNAs (miRNAs) are non-coding small RNAs that regulate gene expression at post-transcriptional level.Thousands of miRNAs have been identified in legumes, but studies about miRNAs linked to peanut nodulefunctionality are scarce. In this work we analyzed transcriptional changes in peanut nodules to identifymiRNAs involved in functional processes of these organs. We found 32 miRNAs precursors differentiallyexpressed in nodules compared with roots, and predicted the potential targets of their corresponding maturemiRNAs. Among them, 20 belong to 14 conserved miRNAs families and 12 are Arachis hypogaea-specificmiRNAs. Expression levels of 3 miRNAs (ahy-miR399, ahy-miR159 and ahy-miR3508) were confirmedexperimentally by qPCR. We also demonstrated that the expression of these miRNAs was not affected byinoculation of a biocontrol bacterium or a fungal pathogen. The catalogue of differentially expressed miRNAprecursors and the expression of the corresponding mature miRNA potential targets in the nodules of A.hypogaea obtained in this work is a database of strong candidates, including A. hypogaea-specific miRNAs, forthe regulation of the nodule functionality. The analysis of their role in this process will certainly lead to thecharacterization of essential regulators in these particular aeschynomenoid nodules.

    • Author Affiliations

       

      MARI´A SOLEDAD FIGUEREDO1 DAMIEN FORMEY2 JOHAN RODRI´GUEZ1 FERNANDO IBA´N˜ EZ1 GEORGINA HERNA´ NDEZ2 ADRIANA FABRA1

      1. Instituto de Investigaciones Agrobiotecnolo´gicas (INIAB)-Consejo Nacional de Investigaciones Cientı´ficas y Tecnolo´gicas (CONICET), Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Fı´sico-quı´micas y Naturales, Universidad Nacional de Rı´o Cuarto, Rı´o Cuarto, 5800 Co´rdoba, Argentina
      2. Centro de Ciencias Geno´micas, Universidad Nacional Auto´noma de Me´xico, 62210 Cuernavaca, Morelos, Mexico
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