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Fourier transform infrared spectroscopy; & alpha; -helical structure; post-traumatic epilepsy

Tissue analysis by Fourier transform infrared (FTIR) imaging can determine the biodistribution of molecules,without pre-analytical modification. We aimed to study the infrared spectroscopic changes of α-helical proteinsat post-traumatic epileptic (PTE) foci by FTIR. FITR mapping was applied to detect α-helical proteins in ratbrain tissue samples with post-traumatic epilepsy. Histological examination of brain sections showed that therat model of PTE was successfully established. At the PTE foci, high α-helical absorption regions wereevident, where the color difference and absorption were significantly different from those in the low-absorptionregions. This provided a distinctive and characteristic pattern at the site of lesions. The use of FTIR imagingmeans that it is possible to measure the molecular structural changes resulting from PTE pathologies in tissues,providing a novel adjunct to conventional pathological diagnostic techniques.

SIYANG XIANG^{1 2} DONG ZHAO^{1 2} HONGXIA HAO¹ XU WANG¹ LING LI³ TIANTONG YANG¹

1. Center of Cooperative Innovation for Judicial Civilization, Beijing 100088, China
2. China University of Political Science and Law, Key Laboratory of Evidence Science, Ministry of Education, Beijing 100088, China
3. Office of the Chief Medical Examiner, State of Maryland, Baltimore, USA

Published

4 April 2020