• Ephedrannin B exerts anti-viral and anti-inflammatory properties in BEAS-2B cells infected with respiratory syncytial virus

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    • Keywords

       

      Ephedrannin B; inflammatory cytokines; respiratory syncytial virus; viral titers

    • Abstract

       

      Ephedrannin B (EPB) has been shown to exert anti-inflammatory effects. However, the effect of EPB onrespiratory syncytial virus infection (RSV) is not known. In this study, the cytotoxic effect of EPB wasevaluated in BEAS-2B cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Reversetranscription quantitative polymerase chain reaction and Western blot assays were performed to determinethe expression of target genes. The anti-viral effect of EPB was assessed by determining viral titers usingplaque assay. We found that RSV infection caused a marked increase in interleukin (IL)-6, IL-8, IL-1beta andtumor necrosis factor (TNF)-alpha production and release, which was concentration-dependently attenuated byEPB treatment. Furthermore, EPB decreased the expression of RSV fusion gene in RSV-infected BEAS-2Bcells. Concomitantly, EPB treatment led to an obvious inhibition of viral replication in BEAS-2B cells.Besides, EPB suppressed RSV-induced mitogen-activated protein kinase/nuclear factor kappa-light-chainenhancerof activated B cells signaling. In conclusion, EPB exerts anti-viral and anti-inflammatory propertiesin BEAS-2B cells infected with RSV.

    • Author Affiliations

       

      SHU HOU1 XIAOYAN XU1 YATING WANG1 YAN YANG2

      1. Department of Pediatrics, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China
      2. Department of Pharmacology, Anhui Medical University, Hefei, China
    • Dates

       
  • Journal of Biosciences | News

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