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      https://www.ias.ac.in/article/fulltext/jbsc/045/0043

    • Keywords

       

      Caspase 3; caspase 8; caspase 9; HCT 116; Hsp70; INT 407; p53; Quinacrine; small GTPases

    • Abstract

       

      Quinacrine (QC), an FDA-approved anti-malarial drug, has shown to have anticancer activities. Due to its

      ‘shotgun’ nature, QC has become an inevitable candidate for combination chemotherapy. There is lack of studyof the molecular interplay between colorectal cancer (CRC) microenvironment and its metastasis. In this study,we focused on the differential anti-cancerous effect of QC on two different human cancer cell lines, HCT 116and INT 407. Results suggest that cytotoxicity increased in both the cell lines with an increase in QCconcentration. The expression patterns of small-GTPases and caspases were altered significantly in QC-treatedcells compared to non-treated cells. HSP70 and p53 showed comparable differences in the expression pattern.The wound-healing assay showed an increase in the denuded zone, with an increase in the concentration ofQC. The formation of apoptotic nuclei increased with a rise in the concentration of QC in both the cell lines.The decrease and increase in caspase 9 and caspase 3 expression respectively were studied, confirmingapoptosis by the extrinsic pathway.

    • Author Affiliations

       

      ANGELA SAMANTA1 GEETHANJALI RAVINDRAN2 ANGSHUMAN SARKAR1

      1. CMBL, Department of Biological Sciences, BITS Pilani K K Birla Goa Campus, Zuarinagar 403 726, India
      2. Centre for Cellular and Molecular Biology, Habsiguda, Uppal Road, Hyderabad 500 007, India
    • Dates

       
  • Journal of Biosciences | News

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