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      https://www.ias.ac.in/article/fulltext/jbsc/045/0040

    • Keywords

       

      HOXB9; prostate cancer; cell cycle; angiogenesis; metastasis

    • Abstract

       

      The Homeobox B9 (HOXB9) is a homeodomain-containing transcription factor that participates in the progressionof various malignancies. Nevertheless, the functional role of HOXB9 in prostate cancer cells is largelyunknown. Hence, we aimed to address the effect of HOXB9 on the progression of prostate cancer cells. Smallinterfering RNA (siRNA) against HOXB9 was used to downregulate HOXB9 expression in PC3 and DU145cells. Western blotting was performed to detect the expression levels of HOXB9 and other related proteins. Cellproliferation was tested by the Cell Counting Kit-8 (CCK-8) and cell cycle and apoptosis were investigated byflow cytometry. Angiogenesis was examined using tube formation assays The Transwell assays were carriedout to assess the migratory and invasive capacities of cells. Here, we found that HOXB9 knockdown significantlyreduced cell proliferation via inducing cell cycle arrest at G1 phase. This treatment also reducedangiogenesis, migration and invasion abilities of PC3 and DU145 cells in vitro. We also found that HOXB9knockdown inhibits the activation of the PI3K/AKT signaling pathway in prostate cancer cells. In conclusion,our findings revealed that HOXB9 promotes prostate cancer progression and might be a novel and effectivetherapeutic target for human prostate cancer.

    • Author Affiliations

       

      HAO XU1 SHANGJUN WU1 XIN SHEN2 DING WU3 ZHENGUO QIN4 HAO WANG4 XIAOGANG CHEN1 XIAOQING SUN4

      1. Department of Urology, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Edong Healthcare Group, Huangshi 435000, Hubei Province, China
      2. Department of Abdominal and Pelvic Medical Oncology II, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Edong Healthcare Group, Huangshi 435000, Hubei Province, China
      3. Department of Urology, Jinling Hospital, Nanjing Medical University, Nanjing 210000, Jiangsu Province, China
      4. Department of Urology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, Jiangsu Province, China
    • Dates

       
  • Journal of Biosciences | News

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      Posted on July 25, 2019

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