• Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/045/0037

    • Keywords

       

      MicroRNA-143–5p; pulmonary artery smooth muscle cells; hypoxic pulmonary hypertension; HIF-1& alpha;

    • Abstract

       

      This paper explores the potential mechanism of microRNA-143–5p regulation effects on pulmonary arterysmooth muscle cells (PASMCs) functions in hypoxic pulmonary hypertension (HPH) via targeting HIF-1α,which may offer a new idea for HPH therapy. PASMCs were transfected with mimics control/miR-143–5pmimics or inhibitor control/miR-143–5p inhibitor. We used Western blotting and RT-qPCR to detect the proteinand mRNA expressions, CCK-8 assay to detect cellular viability, Annexin V-FITC/PI staining and caspase-3/cleaved caspase-3 protein to evaluate cellular apoptosis, transwell migration experiment for cellularmigration measurement and Dual luciferase reporter gene assay to prove the target of miR-143–5p. Cells underhypoxic condition presented the decreased protein and mRNA expressions of a-smooth muscle actin (SM-α-actin),Myocardin, smooth muscle myosin heavy chain (SMMHC), and smooth muscle-22α (SM22α),Calponin1 and Hypoxia-inducible factor-1α (HIF-1α), the increased cell viability and miR-143–5p level; Overexpressionof miR-143–5p obviously reduced vascular smooth muscle-specific contraction marker proteinlevels and cellular apoptosis, increased cellular migration of PASMCs with hypoxia stimulation; Low-expressionof miR-143–5p caused the opposite changes, while co-transfected with Si HIF-1a blocked thebeneficial effects of miR-143–5p inhibition on PASMCs under hypoxia. MicroRNA-143–5p can promote thephenotype conversion, proliferation and migration of pulmonary artery smooth muscle cells under hypoxiccondition through direct targeting of HIF-1α.

    • Author Affiliations

       

      BI TANG1 MING-MING TANG1 QING-MEI XU1 JIAN-LU GUO1 LING XUAN1 JING ZHOU1 XIAO-JING WANG2 HENG ZHANG1 PIN-FANG KANG1

      1. Department of cardiovascular Medicine, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, Anhui, China
      2. Clinical and Basic Provincial Laboratory of Respiratory System Diseases of Anhui Province, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, Anhui, China
    • Dates

       
    • Supplementary Material

       
  • Journal of Biosciences | News

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2021-2022 Indian Academy of Sciences, Bengaluru.