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      https://www.ias.ac.in/article/fulltext/jbsc/045/0031

    • Keywords

       

      Agrobacterium; acetosyringone; embryo axes; Helicoverpa armigera; Spodoptera litura; Cry2Ab; Cry1F; Cry1Ac transcripts

    • Abstract

       

      High-yielding Indian cotton varieties are not amenable for regeneration and transformation because they arerecalcitrant in nature. In this work, we have developed Narasimha (NA1325) cotton variety by introducing three Crygenes driven by three different promoters conferring insect resistance. The meristematic region of embryo axisexplants were infected and co-cultivated with Agrobacterium tumefacience (LBA4404) harbouring pMDC100vector with three Cry gene cassettes (a-globulin : Cry2Ab, DECaMV35s : Cry1F and nodulin : Cry1Ac) with Npt IIas a selectable marker gene. Out of 1010 embryo axes explants infected, 121 (T0) regenerated under two rounds ofkanamycin selectionmedium.About 2551T1 seedswere collected from111T0 plants and these seeds screened againwith kanamycin at seedling stage. The transgenic plants were characterized by PCR, real time quantitative PCR,lateral flow strip protein assay and insect bioassay. Out of 145 kanamycin resistant plants (T1), twelve showedamplification of all four transgenes: Npt II, Cry2Ab, Cry1F and Cry1Ac through PCR with expected amplicons as395, 870, 840 and 618 bp, respectively. Further, lateral flow strip test revealed Cry1F and Cry1Ac proteinsaccumulated in 12 plants, whereas Cry2Ab protein was detected in eight only. The transcripts of all three Cry geneswere accumulated significantly higher in transgenic plants at T2 generation. The transgenic lines showed effectiveresistance againstHelicoverpa armigera and Spodoptera litura larvae. The T2 line L-3 exhibited highest percentageof insect mortality, in which transcripts of all cry genes were accumulated higher than other plants. The transgeniccotton plants carrying triple Cry genes could be an excellent germplasmresource for the breeders for introgressions.

    • Author Affiliations

       

      SUMALATHA KATTA1 2 ASHWINI TALAKAYALA1 MALIREDDY K REDDY3 UMA ADDEPALLY2 MALLIKARJUNA GARLADINNE1

      1. Plant Molecular Biology Laboratory, Agri Biotech Foundation, Rajendranagar, Hyderabad 500 030, India
      2. Center for Biotechnology, Jawaharlal Nehru Technological University, Hyderabad 500 085, India
      3. Crop Improvement Group, International Center for Genetic Engineering and Biotechnology, New Delhi 110 046, India
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