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      https://www.ias.ac.in/article/fulltext/jbsc/044/06/0149

    • Keywords

       

      C-fos-induced growth factor (FIGF); diabetes; histone H3/H4 acetylation; MafA and Ngn3

    • Abstract

       

      Limitation in the number of insulin-producing pancreatic beta-cells is a typical feature of diabetes. It has been indicated thatactivating pancreatic transcription factors can promote the transformation of hepatocytes into insulin-secreting beta-like cells,indicating that direct hepatocyte differentiation seems promising as a treatment for diabetes. Nevertheless, the reprogrammingefficiency still remains low. Our previous study found that the expression of c-fos-induced growth factor (FIGF)was increased in the pancreatic tissues in partial pancreatectomy mice compared to that in normal mice. Here, we observedthat treatment with Ad-FIGF was found to enhance MafA and Ngn3-induced reprogramming of BNL CL.2 cells to b-likecells with the ability of secreting insulin. And FIGF overexpression increased the levels of histone H3/H4 acetylation atMafA and Ngn3 promoter regions in BNL CL.2 cells. Importantly, in vivo study further confirmed that forced expression ofFIGF facilitated the insulin expression and decreased the blood glucose levels in STZ mice. These results strengthen thepossibility of developing cell-based therapies for diabetes through utilizing beta-like cells derived from non-insulin-secretingcells.

    • Author Affiliations

       

      YAQIN HE1 XIAOLIANG XIE2 3 XIAOYAN LI4 SHIKUO RONG3 YUKUI LI5 ZHENHUI LU6

      1. Surgery Laboratory, General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia, China
      2. Department of Colorectal Surgery, General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia, China
      3. College of Clinical Medicine, Ningxia Medical University, Yinchuan 750004, Ningxia, China
      4. Department of Urology, General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia, China
      5. Ningxia Medical University, Yinchuan 750004, Ningxia, China
      6. Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia, China
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  • Journal of Biosciences | News

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