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      https://www.ias.ac.in/article/fulltext/jbsc/044/06/0127

    • Keywords

       

      bglap; bone mineralization; dlx2b; Osterix/sp7; tooth development

    • Abstract

       

      Osterix (or Sp7) is an important transcription factor that promotes osteoblast differentiation by modulating the expression ofa range of target genes. Although many studies have focused on Osterix/Sp7 regulatory mechanisms, the detailed functionshave not been fully elucidated. Toward this end, in this study, we used CRISPR/Cas9 technology to knock out the zebrafishsp7 gene, and then analyzed its phenotype and biological function. Two knockout sp7 mutant lines were successfullyobtained. The bone mineralization level was significantly reduced in the zebrafish sp7-/- homozygote, resulting inabnormal tooth development in the larvae. Quantitative real-time polymerase chain reaction showed that loss of sp7 led todown-regulated expression of the dlx2b and bglap genes related to tooth development and bone mineralization, respectively.Moreover, cell transfection experiments demonstrated that Sp7 directly regulates the expression of dlx2b and bglapthrough Sp7-binding sites on the promoter regions of these two genes. Overall, this study provides new insight into the roleof Sp7 in bone mineralization and tooth development.

    • Author Affiliations

       

      ZHONGJIAN CHEN1 2 3 ZHIYUN SONG3 JINJING YANG4 JIAN HUANG4 HONGBING JIANG1 2

      1. Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China
      2. Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China
      3. The Affiliated Stemmatological Hospital of Soochow University, Suzhou, China
      4. School of Biology and Basic Medical Sciences, Soochow University, Suzhou, China
    • Dates

       
  • Journal of Biosciences | News

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