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    • Keywords

       

      5-Hydroxytryptamine receptor 4; 5-hydroxytryptamine receptor 1b; interferon-alpha; MG132; NH4Cl; p11

    • Abstract

       

      In previous studies, we found interferon-a (IFN-a) could reduce protein levels of p11, 5-hydroxytryptamine receptor 1b(5-HT1b) and 5-hydroxytryptamine receptor 4 (5-HT4), but does not influence their messenger RNA levels in SH-sy5ycells. Thus, we investigated the post-transcriptional modulation of these molecules by IFN-a. SH-sy5y cells were treatedwith IFN-a, NH4Cl or MG132 alone or in combination, and then the protein levels of p11, 5-HT1b and 5-HT4 wereanalyzed by western blots. The regulatory effects of p11 on 5-HT1b and 5-HT4 were also determined in p11 knock-downcells. NH4Cl but not MG132 could reverse the protein level of p11 in IFN-a-treated SH-sy5y cells. MG132 could recoverthe protein levels of 5-HT1b and 5-HT4 in p11 knock-down cells. The down-regulation effects of IFN-a on p11, 5-HT1band 5-HT4 were associated with the lysosome and ubiquitin–proteasome-mediated pathways. p11 was identified as a potentregulator to modulate the ubiquitination of 5-HT1b and 5-HT4. Therefore, it could be potential target therapies in IFN-a induceddepression.

    • Author Affiliations

       

      JIQIANG GUO1 2 HUAXIA DING3 ZHUANGWEI LV2 JUNNA JIAO2 HUI WANG2 YANHONG JI1

      1. Department of Pathogenic Biology and Immunology, School of Basic Medical Sciences, Xi’an Jiaotong University Health Science Centre, Xi’an 710061, People’s Republic of China
      2. Department of Immunology, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, Henan, People’s Republic of China
      3. Third Affiliated Hospital, Xinxiang Medical University, Xinxiang 453003, Henan, People’s Republic of China
    • Dates

       
  • Journal of Biosciences | News

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