• Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/044/04/0100

    • Keywords

       

      ER-alpha; diclofenac; Labisia pumila; osteoarthritis; subchondral bone

    • Abstract

       

      Chondrosenescence (chondrocyte senescence) and subchondral bone deterioration in osteoarthritic rats were analyzed aftertreatment with the estrogenic herb Labisia pumila (LP) or diclofenac. Osteoarthritis (OA) was induced in bilaterallyovariectomized (OVX) rats by injecting mono-iodoacetate into the right knee joints. Rats were grouped (n = 8) into nontreatedOVX?OA control, OVX?OA ? diclofenac (5 mg/kg) (positive control), OVX?OA ? LP leaf extract (150 and300 mg/kg) and healthy sham control. After 8 weeks’ treatment, their conditions were evaluated via serum biomarkers,knee joint histology, bone histomorphometry, protein and mRNA expressions. The LP significantly reduced cartilageerosion, femur bone surface alteration, bone loss and porosity and increased trabecular bone thickness better than diclofenacand the non-treated OA. The cartilage catabolic markers’ (matrix metalloproteinase (MMP)-13, RUNX2, COL10a, ERa,CASP3 and HIF-2a) mRNA expressions were down-regulated and serum bone formation marker, PINP, was increased byLP in a dose-dependent manner. The LP (containing myricetin and gallic acid) showed protection against chondrosenescence,chondrocyte death, hypoxia-induced cartilage catabolism and subchondral bone deterioration. The bone and cartilageprotective effects were by suppressing proteases (collagen break-down), bone resorption and upregulating subchondralbone restoration. The cartilage ERa over-expression showed a strong positive correlation with MMP-13, COL10a1, histological,micro-computed tomography evidence for cartilage degradation and chondrosenescence.

    • Author Affiliations

       

      IFFAH NADHIRA MADZUKI1 2 SENG FONG LAU3 NOR AIJRATUL ASIKIN MOHAMAD SHALAN1 4 NUR ILIYANI MOHD ISHAK1 SUHAILA MOHAMED1

      1. Institute of Bioscience, Universiti Putra Malaysia, UPM Serdang, Malaysia
      2. Faculty of Engineering Technology, Universiti Malaysia Perlis, UniMAP Perlis, Kangar, Malaysia
      3. Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM Serdang, Malaysia
      4. Faculty of Sport Science and Coaching, Universiti Pendidikan Sultan Idris, UPSI, Tanjong Malim, Perak, Malaysia
    • Dates

       
    • Supplementary Material

       
  • Journal of Biosciences | News

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2017-2019 Indian Academy of Sciences, Bengaluru.