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      https://www.ias.ac.in/article/fulltext/jbsc/044/04/0096

    • Keywords

       

      AKT; calycosin; c-Met; glioblastoma; MMP9

    • Abstract

       

      The antitumor effect of calycosin has been widely studied, but the targets of calycosin against glioblastomas are stillunclear. In this study we focused on revealing c-Met as a potential target of calycosin suppressing glioblastomas. In thisstudy, suppressed-cell proliferation and cell invasion together with induced-cell apoptosis appeared in calycosin-treatedU251 and U87 cells. Under treatment of calycosin, the mRNA expression levels of Dtk, c-Met, Lyn and PYK2 wereobserved in U87 cells. Meanwhile a western blot assay showed that c-Met together with matrix metalloproteinases-9(MMP9) and phosphorylation of the serine/threonine kinase AKT (p-AKT) was significantly down-regulated by calycosin.Furthermore, overexpressed c-Met in U87 enhanced the expression level of MMP9 and p-AKT and also improved cellinvasion. Additionally, the expression levels of c-Met, MMP9 and p-AKT were inhibited by calycosin in c-Met overexpressedcells. However, an AKT inhibitor (LY294002) only effected on MMP9 and p-AKT, not on c-Met. These datacollectively indicated that calycosin possibility targeting on c-Met and exert an anti-tumor role via MMP9 and AKT.

    • Author Affiliations

       

      XIAOHU NIE1 YUE ZHOU1 XIAOBING LI1 JIE XU1 XUYAN PAN1 RUI YIN1 BIN LU1

      1. Department of Neurosurgery, Huzhou Central Hospital, Huzhou, Zhejiang, People’s Republic of China
    • Dates

       
  • Journal of Biosciences | News

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      Posted on July 25, 2019

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