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      https://www.ias.ac.in/article/fulltext/jbsc/044/04/0093

    • Keywords

       

      Brown adipose tissue; [18F]FDG; high-fat diet; obesity; PET/CT; RAGE

    • Abstract

       

      Brown adipose tissue (BAT) is responsible for adaptive thermogenesis. We previously showed that genetic deficiency ofreceptor for advanced glycation end products (RAGE) prevented the effects of high-fat diet (HFD). This study was tocompare BAT activity in RAGE knock out (Ager-/-, RKO) and wild-type (WT) mice after treated with HFD or LFD.

      [18F]FDG PET-CT imaging under identical cold-stimulated conditions and mean standard uptake values (SUVmean), ratio ofSUViBAT/SUVmuscle (SUVR, muscle as the reference region) and %ID/g were used for BAT quantification. The resultsshowed that [18F]FDG uptake (e.g., SUVR) in WT-HFD mice was significantly reduced (three-fold) as compared to that inWT-LFD (1.40 ± 0.07 and 4.03 ± 0.38; P = 0.004). In contrast, BAT activity in RKO mice was not significantly affectedby HFD, with SUVRRKO-LFD: 2.14 ± 0.10 and SUVRRKO-LFD: 1.52 ± 0.13 (P = 0.3). The uptake in WT-LFD was almostdouble of that in RKO-LFD (P = 0.004); however, there was no significant difference between RKO-HFD and WT-HFDmice (P = 0.3). These results, corroborating our previous findings on the measurement of mRNA transcripts for UCP1 inthe BAT, suggest that RAGE may contribute to altered energy expenditure and provide a protective effect against HFD byAger deletion (Ager -/-).

    • Author Affiliations

       

      YU-SHIN DING1 2 NOEEN MALIK1 SEBASTIAN MENDOZA1 DANIEL TUCHMAN1 CARMEN HURTADO DEL POZO3 RAQUEL LOPEZ DIEZ3 ANN-MARIE SCHMIDT3

      1. Department of Radiology, New York University School of Medicine, New York, USA
      2. Department of Psychiatry, New York University School of Medicine, New York, USA
      3. Diabetes Research Program, Division of Endocrinology, Department of Medicine, New York University School of Medicine, New York, USA
    • Dates

       
  • Journal of Biosciences | News

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