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      https://www.ias.ac.in/article/fulltext/jbsc/044/04/0081

    • Keywords

       

      Biosafety assessment; Oryza sativa; proteomics; rice stripe virus; transgenic rice; transcriptomics; virus resistance

    • Abstract

       

      Stable transgenic rice line (named KRSV-1) with strong resistance against rice stripe virus was generated using the genesequence of disease-specific protein by RNA interference. Comprehensive safety assessment of transgenic plants has turnedinto a significant field of genetic modification food safety. In this study, a safety assessment of KRSV-1 was carried out in astepwise approach. The molecular analysis exhibited that KRSV-1 harbored one copy number of transgene, which wasintegrated into the intergenic non-coding region of chromosome 2 associated with inter-chromosomal translocations of 1.6-kb segments of chromosome 8. Then, transcriptomics and proteomics analyses were carried out to detect the unintendedeffects as a result of the integration of the transgene. Although 650 dramatically differentially expressed genes (DDEGs)and 357 differentially expressed proteins were detected between KRSV-1 and wild-type (WT) by transcriptomics andproteomics analyses, no harmful members in the form of toxic proteins and allergens were observed. Encouragingly, thenutritional compositions of seeds from KRSV-1 were comparable with WT seeds. The results of this entire study ofmolecular analysis, transcriptome and proteome profile of KRSV-1 revealed that no detrimental changes in the form of toxicproteins and allergens were detected in the transgenic rice line due to the integration of the transgene.

    • Author Affiliations

       

      YANG XU1 2 LVJIE BI1 ZIPENG YU1 CHAO LIN3 LIMING GAN1 LIFEI ZHU1 HAIYANG LI1 YUNZHI SONG1 CHANGXIANG ZHU1

      1. State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an 271018, Shandong, People’s Republic of China
      2. Shandong Peanut Research Institute, Shandong Academy of Agricultural Sciences, Qingdao 266100, Shandong, People’s Republic of China
      3. ShanDong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266500, Shandong, People’s Republic of China
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