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      https://www.ias.ac.in/article/fulltext/jbsc/044/04/0080

    • Keywords

       

      Apoptosis; bladder cancer; miR-212-3p; NFIA; proliferation

    • Abstract

       

      Accumulating evidence suggest that microRNAs play crucial roles in the development and progression of bladder cancer(BC). Here, we found that miR-212-3p was significantly down-regulated and negatively correlated with nuclear factor IA(NFIA) in human BC tissues. Bioinformatics analysis predicted that NFIA was a target gene of miR-212-3p. Then BC celllines, T24 and J82 cells were transfected with miR-212-3p mimics or siNFIA to obtain miR-212-3p overexpression or NFIAknockdown cell lines, respectively. Quantitative real-time PCR was used to determine the expression of miR-212-3p andNFIA. Western blot analysis was utilized to detect NFIA expression. MTT assay showed either miR-212-3 overexpression orNFIA knockdown significantly inhibited the BC cell proliferation. Double staining with Annexin V-APC and 7-AAD showedthe total number of apoptotic BC cells were remarkably increased after miR-212-3p overexpression or NFIA knockdown.Collectively, our results indicated that miR-212-3p targeting NFIA might serve as a promising target for BC.

    • Author Affiliations

       

      XIAOMING WU1 HAO CHEN1 GAOYUE ZHANG1 JIANHUI WU1 WEI ZHU1 YANQIN GU1 YI HE1

      1. Department of Urology, the First Hospital of Jiaxing, Jiaxing, Zhejiang Province, People’s Republic of China
    • Dates

       
  • Journal of Biosciences | News

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