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      https://www.ias.ac.in/article/fulltext/jbsc/044/02/0036

    • Keywords

       

      dFUS; hnRNPs; kuz; mmp2; SPARC; TDP-43; Trithorax

    • Abstract

       

      We examined interactions between the 83 kDa heat-shock protein (Hsp83) and hsrω long noncoding RNAs (lncRNAs) in hsrω66 Hsp90GFP homozygotes, which almost completely lack hsrω lncRNAs but over-express Hsp83. All +/+; hsrω66 Hsp90GFP progeny died before the third instar. Rare Sp/CyO; hsrω66 Hsp90GFP reached the third instar stage but phenocopied l(2)gl mutants, becoming progressively bulbous and transparent with enlarged brain and died after prolonged larval life. Additionally, ventral ganglia too were elongated. However, hsrω66 Hsp90GFP/TM6B heterozygotes, carrying +/ + or Sp/CyO second chromosomes, developed normally. Total RNA sequencing (+/+, +/+; hsrω66/hsrω66, Sp/CyO; hsrω66/ hsrω66, +/+; Hsp90GFP/Hsp90GFP and Sp/CyO; hsrω66 Hsp90GFP/hsrω66 Hsp90GFP late third instar larvae) revealed similar effects on many genes in hsrω66 and Hsp90GFP homozygotes. Besides additive effect on many of them, numerous additional genes were affected in Sp/CyO; hsrω66 Hsp90GFP larvae, with l(2)gl and several genes regulating the central nervous system being highly down-regulated in surviving Sp/CyO; hsrω66 Hsp90GFP larvae, but not in hsrω66 or Hsp90GFP single mutants. Hsp83 and several omega speckle-associated hnRNPs were bioinformatically found to potentially bind with these gene promoters and transcripts. Since Hsp83 and hnRNPs are also known to interact, elevated Hsp83 in an altered background of hnRNP distribution and dynamics, due to near absence of hsrω lncRNAs and omega speckles, can severely perturb regulatory circuits with unexpected consequences, including down-regulation of tumoursuppressor genes such as l(2)gl.

    • Author Affiliations

       

      MUKULIKA RAY1 2 SUNDARAM ACHARYA1 3 SAKSHI SHAMBHAVI1 4 SUBHASH C LAKHOTIA1

      1. Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221005, India
      2. Present Address: Department of Molecular Biology, Cellular Biology and Biochemistry, Brown University, Providence, USA
      3. Present Address: CSIR-Institute of Genomics and Integrative Biology, Mathura Road, Delhi 110025, India
      4. Present Address: Centre for Cellular and Molecular Biology, Hyderabad 500007, India
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