• Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/044/01/0017

    • Keywords

       

      Autophagy; Atg8; autophagosome biogenesis; ERES; Tip20

    • Abstract

       

      Autophagy is a highly conserved intracellular degradation pathway in eukaryotic cells that responds to environmentalchanges. Genetic analyses have shown that more than 40 autophagy-related genes (ATG) are directly involved in thisprocess in fungi. In addition to Atg proteins, most vesicle transport regulators are also essential for each step of autophagy.The present study showed that one Endoplasmic Reticulum protein in Saccharomyces cerevisiae, Tip20, which controlsGolgi-to-ER retrograde transport, was also required for starvation-induced autophagy under high temperature stress. Intip20 conditional mutant yeast, the transport of Atg8 was impaired during starvation, resulting in multiple Atg8 punctadispersed outside the vacuole that could not be transported to the pre-autophagosomal structure/phagophore assembly site(PAS). Several Atg8 puncta were trapped in ER exit sites (ERES). Moreover, the GFP-Atg8 protease protection assayindicated that Tip20 functions before autophagosome closure. Furthermore, genetic studies showed that Tip20 functionsdownstream of Atg5 and upstream of Atg1, Atg9 and Atg14 in the autophagy pathway. The present data show that Tip20,as a vesicle transport regulator, has novel roles in autophagy.

    • Author Affiliations

       

      LEI CHEN1 CHUNLING ZHANG1 YUANCUN LIANG1 AIXIN LIU1 HANSONG DONG1 SHENSHEN ZOU1

      1. Department of Plant Pathology, Shandong Agricultural University, Tai’an 271018, China
    • Dates

       
  • Journal of Biosciences | News

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2017-2019 Indian Academy of Sciences, Bengaluru.