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      https://www.ias.ac.in/article/fulltext/jbsc/043/05/0969-0983

    • Keywords

       

      AMPK; diabetes; GLUT4; MDLE treatment; PPARAMPK; diabetes; GLUT4; MDLE treatment; PPARAMPK; diabetes; GLUT4; MDLE treatment; PPAR𝛾;

    • Abstract

       

      The study was designed to explore the beneficial effect of Musca domestica larvae extract (MDLE) on a metabolic disorderusing a diabetic rat model. Streptozotocin-induced diabetic rats were treated with or without MDLE. Blood glucose, insulinlevels, lipid profiles, and oxidative stress markers were measured. The morphological changes in the pancreas and liverwere determined, as well as insulin expression. The expression of glucose transporter 4 (GLUT4), phospho-adenosinemonophosphate-activated protein kinase (p-AMPK)/total AMPK, superoxide dismutase 1 (SOD1), catalase (CAT), andperoxisome proliferator-activated receptor gamma (PPAR𝛾) were detected. Compared with untreated diabetic rats, MDLEtreatedrats had decreased urine volume, food intake, and water intake, along with significantly lower levels of bloodglucose, malondialdehyde (MDA), plasma triglycerides, low-density lipoprotein (LDL), and total cholesterol. MDLE-treatedrats also had higher levels of SOD activity, high-density lipoprotein (HDL), and insulin. MDLE treatment partiallyrestored the β-cell population, improved the liver necrosis and islet cell damage, reversed the decreased expression ofGLUT4, phospho-AMPK, SOD1, and CAT in the liver, skeletal muscle and pancreatic tissue, and also increased theexpression of PPAR𝛾 in the liver and adipose tissue in diabetic rats. In conclusion, the obtained results suggest that MDLEcould possibly be used pharmacologically as an adjuvant for the treatment of diabetes.

    • Author Affiliations

       

      HANFANG MEI1 2 3 JIANHUA XU4 YINRU HE2 XIA YANG2 WENBIN LIU1 2 WEI TIAN2 YU ZENG5 JIAYONG ZHU1

      1. Guangdong Key Laboratory of Pharmaceutical Bioactive Substances, Guangzhou 510006, Guangdong, China
      2. School of Basic Courses, Guangzhou 510006, Guangdong, China
      3. Department of Biochemistry and Molecular Biology, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou 510006, Guangdong, China
      4. Department of Laboratory Science, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510120, Guangdong, China
      5. Key Laboratory of Digital Quality Evaluation of Chinese Material Medica of ASTCM, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou 510006, Guangdong, China
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