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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/043/05/0947-0957

    • Keywords

       

      CSFV; interactive cellular protein; NS4B; TBK1

    • Abstract

       

      Classical swine fever (CSF) is a contagious disease with a high mortality rate and is caused by classical swine fever virus(CSFV). CSFV non-structural protein 4B (NS4B) plays a crucial role in CSFV replication and pathogenicity. However,precisely how NS4B exerts these functions remains unknown, especially as there are no reports relating to potential cellularpartners of CSFV NS4B. Here, a yeast two-hybrid (Y2H) system was used to screen the cellular proteins interacting withNS4B from a porcine alveolar macrophage (PAM) cDNA library. The protein screen along with alignment using the NCBIdatabase revealed 14 cellular proteins that interact with NS4B: DDX39B, COX7C, FTH1, MAVS, NR2F6, RPLP1,PSMC4, FGL2, MKRN1, RPL15, RPS3, RAB22A, TP53BP2 and TBK1. These proteins mostly relate to oxidoreductaseactivity, signal transduction, localization, biological regulation, catalytic activity, transport and metabolism by GO categories.Tank-binding kinase 1 (TBK1) was chosen for further confirmation. The NS4B-TBK1 interaction was furtherconfirmed by subcellular co-location, co-immunoprecipitation and glutathione S-transferase pull-down assays. This studyoffers a theoretical foundation for further understanding of the diversity of NS4B functions in relation to viral infection andsubsequent pathogenesis.

    • Author Affiliations

       

      HUIFANG LV1 2 WANG DONG1 3 ZHI CAO1 JIHUI LIN1 YUELING OUYANG1 KANGKANG GUO1 CUNFA LI2 YANMING ZHANG1

      1. College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling 712100, Shaanxi, China
      2. College of Pharmaceutical Engineering, Henan University of Animal Husbandry and Economy, No. 6 Longzihu Northern Road, Zhengzhou 450046, Henan, China
      3. College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, No. 6 Longzihu Northern Road, Zhengzhou 450046, Henan, China

    • Dates

       
      Published
      1 December 2018

  • Journal of Biosciences | News

      Forthcoming Special issue.


    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

      Posted on April 12, 2023
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    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

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