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      https://www.ias.ac.in/article/fulltext/jbsc/043/05/0911-0919

    • Keywords

       

      AKT2; MiR-92a; rheumatoid arthritis; RA-FLS

    • Abstract

       

      Growing data have indicated that the miR-17–92 cluster is implicated in inflammatory response and rheumatoid arthritis(RA). This study was aimed to investigate the effects of miR-92a on the proliferation and migration of rheumatoid arthritisfibroblast-like synoviocytes (RA-FLSs). Our results showed that miR-92a was significantly down-regulated in RA synovialtissue and RA-FLSs, whereas the protein level of AKT2 is increased. Restoration of miR-92a suppressed the proliferationand migration of RA-FLSs. Down-regulation of miR-92a promotes proliferation and migration of normal human FLSs.Dual luciferase reporter gene assay showed that miR-92a could specifically bind with the 30UTR of AKT2 and significantlyrepressed the luciferase activity. Down-regulation or up-regulation of miR-92a significantly increased or decreased theprotein and phosphorylation levels of AKT2. siRNA-mediated down-regulation of AKT2 significantly prevented cellproliferation and migration of RA-FLSs, which were similar to the effects induced by overexpression of miR-92a.Moreover, AKT2 overexpression rescued miR-92a-mediated suppressive effect on proliferation and migration of RA-FLS.Thus, miR-92a could inhibit the proliferation and migration of RA-FLSs through regulation of AKT2 expression.

    • Author Affiliations

       

      FANG-YUAN YU1 CONG-QIN XIE1 CHANG-LIANG JIANG1 JI-TONG SUN1 HUI-CHENG FENG1 CHAO LI1 XUN-WU HUANG1

      1. Department of Orthopaedics, 309th Hospital of Chinese PLA, Beijing 100091, People’s Republic of China
    • Dates

       
  • Journal of Biosciences | News

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