Decreased PGC1-α levels and increased apoptotic protein signaling are associated with the maladaptive cardiac hypertrophy in hyperthyroidism
RAYANE BRINCK TEIXEIRA TATIANE EVELYN BARBOZA CARLA CRISTINA DE ARAU´ JO RAFAELA SIQUEIRA ALEXANDRE LUZ DE CASTRO JE´ SSICA HELLEN POLETTO BONETTO BRUNA GAZZI DE LIMA-SEOLIN CRISTINA CAMPOS CARRARO ADRIANE BELLO´ -KLEIN PAWAN K SINGAL ALEX SANDER DA ROSA ARAUJO
Hyperthyroidism can lead to the activation of proteins which are associated with inflammation, apoptosis, hypertrophy, and heart failure. This study aimed to explore the inflammatory and apoptotic proteins involved in the hyperthyroidism-inducedcardiac hypertrophy establishment. Male Wistar rats were divided into control and hyperthyroid (12 mg/L L-thyroxine, indrinking water for 28 days) groups. The expression of inflammatory and apoptotic signaling proteins was quantified in the left ventricle by Western blot. Hyperthyroidism was confirmed by evaluation of T3 and T4 levels, as well as cardiac hypertrophy development. There was no change in the expression of HSP70, HIF1-α, TNF-α, MyD88, p-NFκB, NFκB,p-p38, and p38. Reduced expression of p53 and PGC1-α was associated with increased TLR4 and decreased IL-10expression. Decreased Bcl-2 expression and increased Bax/Bcl-2 ratio were also observed. The results suggest that reducedPGC1-a and IL-10, and elevated TLR4 proteins expression could be involved with the diminished mitochondrial biogenesisand anti-inflammatory response, as well as cell death signaling, in the establishment of hyperthyroidism-inducedmaladaptive cardiac hypertrophy.
RAYANE BRINCK TEIXEIRA1 TATIANE EVELYN BARBOZA2 CARLA CRISTINA DE ARAU´ JO1 RAFAELA SIQUEIRA1 ALEXANDRE LUZ DE CASTRO1 JE´ SSICA HELLEN POLETTO BONETTO1 BRUNA GAZZI DE LIMA-SEOLIN1 CRISTINA CAMPOS CARRARO1 ADRIANE BELLO´ -KLEIN1 PAWAN K SINGAL3 ALEX SANDER DA ROSA ARAUJO1
Volume 45, 2020
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