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    • Fluorescence spectroscopy for revealing mechanisms in biology: Strengths and pitfalls

      G KRISHNAMOORTHY

      Article Volume 43 Issue 3 July 2018 pp 555-567

    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/043/03/0555-0567

    • Keywords

       

      Fluorescence; fluorescence anisotropy; fluorescence lifetime; fluorescence quenching; Maximum Entropy Method; sample heterogeneity; time-resolved fluorescence

    • Abstract

       

      This article describes the basic principles of steady-state and time-resolved fluorescence. The formal equivalence of the twomethodologies is described first, followed by the extra advantages of time-resolved methods in revealing populationheterogeneity in complex systems encountered in biology. Several examples from the author’s work are described insupport of the above contention. Finally, several misinterpretations and pitfalls in the interpretation of fluorescence data andtheir remedy are described.

    • Author Affiliations

       

      G KRISHNAMOORTHY1 2

      1. Department of Biotechnology, Anna University, Chennai 600 025, India
      2. Department of Chemistry, Indian Institute of Technology, Mumbai 400 076, India

    • Dates

       
      Published
      1 July 2018

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