• Fluorescence spectroscopy for revealing mechanisms in biology: Strengths and pitfalls

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    • Keywords


      Fluorescence; fluorescence anisotropy; fluorescence lifetime; fluorescence quenching; Maximum Entropy Method; sample heterogeneity; time-resolved fluorescence

    • Abstract


      This article describes the basic principles of steady-state and time-resolved fluorescence. The formal equivalence of the twomethodologies is described first, followed by the extra advantages of time-resolved methods in revealing populationheterogeneity in complex systems encountered in biology. Several examples from the author’s work are described insupport of the above contention. Finally, several misinterpretations and pitfalls in the interpretation of fluorescence data andtheir remedy are described.

    • Author Affiliations



      1. Department of Biotechnology, Anna University, Chennai 600 025, India
      2. Department of Chemistry, Indian Institute of Technology, Mumbai 400 076, India
    • Dates

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