• Fluorescence spectroscopy for revealing mechanisms in biology: Strengths and pitfalls

    • Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/043/03/0555-0567

    • Keywords

       

      Fluorescence; fluorescence anisotropy; fluorescence lifetime; fluorescence quenching; Maximum Entropy Method; sample heterogeneity; time-resolved fluorescence

    • Abstract

       

      This article describes the basic principles of steady-state and time-resolved fluorescence. The formal equivalence of the twomethodologies is described first, followed by the extra advantages of time-resolved methods in revealing populationheterogeneity in complex systems encountered in biology. Several examples from the author’s work are described insupport of the above contention. Finally, several misinterpretations and pitfalls in the interpretation of fluorescence data andtheir remedy are described.

    • Author Affiliations

       

      G KRISHNAMOORTHY1 2

      1. Department of Biotechnology, Anna University, Chennai 600 025, India
      2. Department of Chemistry, Indian Institute of Technology, Mumbai 400 076, India
    • Dates

       
  • Journal of Biosciences | News

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2017-2019 Indian Academy of Sciences, Bengaluru.