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    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/043/03/0519-0540

    • Keywords

       

      Fluorescence correlation spectroscopy; Fo¨ rster resonance energy transfer; single molecule fluorescence; single molecule virology; single particle tracking; super-resolution microscopy

    • Abstract

       

      Single-molecule fluorescence methods remain a challenging yet information-rich set of techniques that allow one to probethe dynamics, stoichiometry and conformation of biomolecules one molecule at a time. Viruses are small (nanometers) insize, can achieve cellular infections with a small number of virions and their lifecycle is inherently heterogeneous with alarge number of structural and functional intermediates. Single-molecule measurements that reveal the complete distributionof properties rather than the average can hence reveal new insights into virus infections and biology that are inaccessibleotherwise. This article highlights some of the methods and recent applications of single-molecule fluorescence in the field ofvirology. Here, we have focused on new findings in virus–cell interaction, virus cell entry and transport, viral membranefusion, genome release, replication, translation, assembly, genome packaging, egress and interaction with host immuneproteins that underline the advantage of single-molecule approach to the question at hand. Finally, we discuss the challenges,outlook and potential areas for improvement and future use of single-molecule fluorescence that could further aidour understanding of viruses.

    • Author Affiliations

       

      SUNAINA BANERJEE1 SATYAGHOSH MAURYA2 RAHUL ROY1 2 3

      1. Molecular Biophysics Unit, Indian Institute of Science, Bengaluru, India
      2. Department of Chemical Engineering, Indian Institute of Science, Bengaluru, India
      3. Centre for Biosystems Science and Engineering, Indian Institute of Science, Bengaluru, India

    • Dates

       
      Published
      1 July 2018

  • Journal of Biosciences | News

      Forthcoming Special issue.


    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

      Posted on April 12, 2023
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      Posted on July 25, 2019

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