• Fluorescence-based approaches for monitoring membrane receptor oligomerization

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      https://www.ias.ac.in/article/fulltext/jbsc/043/03/0463-0469

    • Keywords

       

      Anisotropy; fluorescence lifetime imaging microscopy; fluorescence; image correlation spectroscopy; membrane; receptor

    • Abstract

       

      Membrane protein structures are highly under-represented relative to water-soluble protein structures in the protein databank.This is especially the case because membrane proteins represent more than 30% of proteins encoded in the humangenome yet contribute to less than 10% of currently known structures (Torres et al. in Trends Biol Sci 28:137–144, 2003).Obtaining high-resolution structures of membrane proteins by traditional methods such as NMR and x-ray crystallographyis challenging, because membrane proteins are difficult to solubilise, purify and crystallize. Consequently, development ofmethods to examine protein structure in situ is highly desirable. Fluorescence is highly sensitive to protein structure anddynamics (Lakowicz in Principles of fluorescence spectroscopy, Springer, New York, 2007). This is mainly because of thetime a fluorescence probe molecule spends in the excited state. Judicious choice and placement of fluorescentmolecule(s) within a protein(s) enables the experimentalist to obtain information at a specific site(s) in the protein (complex)of interest. Moreover, the inherent multi-dimensional nature of fluorescence signals across wavelength, orientation, spaceand time enables the design of experiments that give direct information on protein structure and dynamics in a biologicalsetting. The purpose of this review is to introduce the reader to approaches to determine oligomeric state or quaternarystructure at the cell membrane surface with the ultimate goal of linking the oligomeric state to the biological function. In thefirst section, we present a brief overview of available methods for determining oligomeric state and compare theiradvantages and disadvantages. In the second section, we highlight some of the methods developed in our laboratory toaddress contemporary questions in membrane protein oligomerization. In the third section, we outline our approach todetermine the link between protein oligomerization and biological activity.

    • Author Affiliations

       

      ANDREW HA CLAYTON1

      1. Cell Biophysics Laboratory, Centre for Micro-Photonics, Department of Physics and Astronomy, School of Science, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Melbourne, Australia
    • Dates

       
  • Journal of Biosciences | News

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