1. Home
    2. Journals
    3. Journal of Biosciences
    4. Volume 43
    5. Issue 3

    • A novel fluorescence microscopic approach to quantitatively analyse protein-induced membrane remodelling

      THOMAS J PUCADYIL

      Article Volume 43 Issue 3 July 2018 pp 431-435

    • Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/043/03/0431-0435

    • Keywords

       

      Fluorescence microscopy; membrane remodelling; membrane tubes

    • Abstract

       

      Membrane remodelling or the bending and rupture of the lipid bilayer occurs during diverse cellular processes such as celldivision, synaptic transmission, vesicular transport, organelle biogenesis and sporulation. These activities are brought aboutby the localized change in membrane curvature, which in turn causes lipid-packing stress, of a planar lipid bilayer byproteins. For instance, vesicular transport processes are typically characterized by the cooperative recruitment of proteinsthat induce budding of a planar membrane and catalyse fission of the necks of membrane buds to release vesicles. Theanalysis of such membrane remodelling reactions has traditionally been restricted to electron microscopy–based approachesor force spectroscopic analysis of membrane tethers pulled from liposome-based model membrane systems. Our recentwork has demonstrated the facile creation of tubular model membrane systems of supported membrane tubes (SMrTs),which mimic late-stage intermediates of typical vesicular transport reactions. This review addresses the nature of such anassay system and a fluorescence-intensity-based analysis of changes in tube dimensions that is indicative of the membraneremodelling capacity of proteins.

    • Author Affiliations

       

      THOMAS J PUCADYIL1

      1. Indian Institute of Science Education and Research, Dr. Homi Bhabha Road, Pashan, Pune 411 008, India

    • Dates

       
      Published
      1 July 2018

  • Journal of Biosciences | News

      Forthcoming Special issue.


    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

      Posted on April 12, 2023
      <

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2023-2024 Indian Academy of Sciences, Bengaluru.

Contact | Site index