A novel fluorescence microscopic approach to quantitatively analyse protein-induced membrane remodelling
Membrane remodelling or the bending and rupture of the lipid bilayer occurs during diverse cellular processes such as celldivision, synaptic transmission, vesicular transport, organelle biogenesis and sporulation. These activities are brought aboutby the localized change in membrane curvature, which in turn causes lipid-packing stress, of a planar lipid bilayer byproteins. For instance, vesicular transport processes are typically characterized by the cooperative recruitment of proteinsthat induce budding of a planar membrane and catalyse fission of the necks of membrane buds to release vesicles. Theanalysis of such membrane remodelling reactions has traditionally been restricted to electron microscopy–based approachesor force spectroscopic analysis of membrane tethers pulled from liposome-based model membrane systems. Our recentwork has demonstrated the facile creation of tubular model membrane systems of supported membrane tubes (SMrTs),which mimic late-stage intermediates of typical vesicular transport reactions. This review addresses the nature of such anassay system and a fluorescence-intensity-based analysis of changes in tube dimensions that is indicative of the membraneremodelling capacity of proteins.
Volume 46, 2020
Continuous Article Publishing mode
Click here for Editorial Note on CAP Mode