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      https://www.ias.ac.in/article/fulltext/jbsc/043/02/0351-0364

    • Keywords

       

      Autophagy; development; Dictyostelium; patterning; sir2A

    • Abstract

       

      Sirtuins are a family of deacetylases (Class III histone deacetylases) with evolutionarily conserved functions in cellularmetabolism and chromatin regulation. Out of the seven human Sirtuins, the function of Sirt2 is the least understood. Thepurpose of the present study was to investigate the role of Sir2A, a homolog of human Sirt2 in Dictyostelium discoideum(Dd), a lower eukaryote. We created both overexpressing and deletion strains of Ddsir2A to analyse its functions. Weobserved sir2A mRNA expression throughout development and the transcript was present in the prespore/spore region ofmulticellular structures developed. They show a preference towards prestalk/stalk pathway when co-developed with wildtypecells during chimera formation. Deletion strain showed a multi-tipped phenotype, decrease in cell proliferation andinhibition of autophagy. In conclusion, our results show low cAMP levels, reduced cell-adhesion, weak cell migration andimpaired autophagy to be responsible for the phenotype shown by the null cells. This study provides new insights into thefunctions of Ddsir2A.

    • Author Affiliations

       

      RAKHEE LOHIA1 2 PUNITA JAIN1 3 MUKUL JAIN1 HIMANSHU MISHRA1 PRADEEP KUMAR BURMA2 ANJU SHRIVASTAVA3 SHWETA SARAN1

      1. School of Life Sciences, Jawaharlal Nehru University, New Delhi 110 067, India
      2. Department of Genetics, University of Delhi, South Campus, New Delhi 110 021, India
      3. Department of Zoology, University of Delhi, New Delhi 110 007, India
    • Dates

       
    • Supplementary Material

       
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